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Human FANCE knockdown cell line

TypeKnockdown(KD) Stable Cell Line
SpeciesHomo sapiens
Biosafety Level1
Size1 × 10^6 cells / vial
StorageLiquid Nitrogen
ShippingDry Ice
Growth PropertiesHuman FANCE knockdown cell line needs to be cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Quality ControlGenotype: FANCE knockdown; Validation of gene knockdown is performed using real-time qRT-PCR (mRNA expression level) or WB (protein expression level).
Host CellHEK293 or HeLa(other host cells are also available)
GeneinfoFACE, FAE
Recommended MediumGrowth Medium: IMDM, 10% FCS; Freeze Medium: IMDM, 20% FCS, 10% DMSO; Revival: Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish.
DescriptionHuman FANCE knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. AcceGen Biotech offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.
Citation GuideIf the product is used for publication, it should be cited in the publication as: “AcceGen Biotech Cat.# XXX-0000”. In addition,click here to submit your paper’s PubMed ID (PMID) to get a $100 coupon.
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