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Human ZYX knockout cell line

TypeKnockout Stable Cell Line
SpeciesHomo sapiens
Biosafety Level1
StorageLiquid Nitrogen
ShippingDry Ice
Growth PropertiesCells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Quality ControlGenotype: ZYX HEK293/HeLa knockout
Host CellHEK293/HeLa
GeneinfoESP-2, HED-2
Recommended MediumGrowth Medium: IMDM, 10% FCS; Freeze Medium: IMDM, 20% FCS, 10% DMSO; Revival: Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish.
DescriptionHuman ZYX knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.
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