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T4 DNA Polymerase

Cat.#AGE-0006
ComponentT4 DNA Polymerase (5,000 U/ml); 10X ABuffer B
Size200 U / 1000 U
StorageStorage Conditions: 100 mM KPO4, 1 mM DTT, 50% Glycerol, pH 6.5 @ 25°C. Store the T4 DNA Polymerase at -20°C. Please avoid repeated freeze-thaw cycles.
DescriptionT4 DNA Polymerase catalyzes the synthesis of DNA in the 5'→3' direction and requires the presence of template and primer. This enzyme has a 3'→5' exonuclease activity which is much more active than that found in DNA Polymerase I (E. coli). Unlike E. coli DNA Polymerase I, T4 DNA Polymerase does not have a 5'→3' exonuclease function. It is applicable to 3´ overhang removal to form blunt ends, 5´ overhang fill-in to form blunt ends, single strand deletion subcloning, second strand synthesis in site-directed mutagenesis and probe labeling using replacement synthesis. Theoretical protein molecular weight is 104000 daltons. Error Rate: ~ 1x10-6 bases
Reaction Conditions1X ABuffer B, Incubate at 12°C
ApplicationBlunting, PCR, Polymerases for DNA Manipulation
Citation GuideIf the product is used for publication, it should be cited in the publication as: “AcceGen Biotech Cat.# XXX-0000”. In addition,click here to submit your paper’s PubMed ID (PMID) to get a $100 coupon.
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