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T4 DNA Ligase (High Concentration)

ComponentT4 DNA Ligase (High Conc.); 10X T4 DNA ligase Reaction Buffer
Size80000 U / 400000 U
StorageStorage Conditions: 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH7.4 @ 25°C. Store the T4 DNA Ligase (High Conc.) at -20°C. Please avoid repeated freeze-thaw cycles.
DescriptionT4 DNA Ligase can catalyze the formation of a phosphodiester bond between juxtaposed 5' phosphate and 3' hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA and some DNA/RNA hybrids. T4 DNA ligase will seal nicks for these DNA substrates. Applicable to Cloning of restriction fragments, joining linkers and adapters to blunt-ended DNA.
ApplicationCloning Ligation, Adaptor Ligation
Citation GuideIf the product is used for publication, it should be cited in the publication as: “AcceGen Biotech Cat.# XXX-0000”. In addition,click here to submit your paper’s PubMed ID (PMID) to get a $100 coupon.
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