For research use only
| Cat No. | ABL-TC0252 |
| Product Type | Human Cervial Cell Lines |
| Cell Type | Epithelial |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Cervix |
| Product Code | HeLa 9903; Hela-9903 |
HeLa9903 Cell Line transfected with estrogen receptor-alpha reporter construct provides models for hormone signaling and cancer biology investigations.
HeLa9903 is a human cervical carcinoma cell line derived from parental HeLa cells and genetically engineered for enhanced analysis of estrogen receptor alpha (ERα) signaling, reflecting the well-documented HeLa9903 origin and history as an ERα-responsive reporter model. The cells exhibit typical epithelial morphology and adherent growth. HeLa9903 stably expresses ERα via non-viral transfection together with an estrogen response element (ERE)-driven luciferase reporter under the control of a mouse metallothionein promoter, enabling sensitive and real-time quantification of ERα transcriptional activity and supporting HeLa9903 gene expression studies focused on hormone-responsive signaling pathways. The cell line maintains a hypertriploid karyotype with multiple chromosomal abnormalities and expresses high levels of HPV18 E6 and E7 oncogenes, which drive cell cycle dysregulation and cellular immortalization. Its tumorigenic and metastatic potential has been confirmed in xenograft systems, making HeLa9903 a robust platform among HeLa9903 experimental models for investigating ERα signaling, HPV-associated oncogenesis, and therapeutic responses in cervical cancer research.
| Product Code | HeLa 9903; Hela-9903 |
| Species | Human |
| Cat.No | ABL-TC0252 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Cervix |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Cervial Cell Lines |
HeLa9903 cells are a valuable model for studying estrogen receptor (ER) α transactivation, enabling evaluation of chemical binding affinities and agonist activities, including bisphenol A and phyto- and mycoestrogens. They also serve as a key system for investigating HPV18-driven cervical carcinogenesis, tumor progression, and mechanisms of cell cycle regulation, metastasis, and chemoresistance. Their consistent growth and genetic stability make them ideal for translational oncology and antiviral drug screening.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
The HeLa9903 is HeLa cell line that is stably expressing human estrogen receptor-alpha, reportor gene (estrogen response element linked to luciferase), and firefly luciferase gene (pGL3(Luc+)).
The introduced plasmids include
pGL3-TATA-EREx5-BSD: reportor gene (upstream sequences of Xenopus-derived vitronectin gene containing estrogen responce element was linked to firefly luciferase gene (Luc+))
pRC/RSV-hERalpha Kozak: human estrogen receptor alpha gene driven by RSV promotor.
DMEM with 10% heat inactivated fetal bovine serum
