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MicroRNA Agomir/Antagomir Synthesis

AcceGen has pioneered the development of microRNA research and diagnostics tools with leading-edge services. We’re committed to paving new ways to meet all your needs for miRNA agomir/antagomir synthesis.

What is MicroRNA Agomir/Antagomir?

Agomir is chemically-modified double-strand miRNA mimic with modified antisense strand: 2 phosphorothioates at the 5′ end, 4 phosphorothioates at the 3′ end, 3′ end cholesterol group, and full-length nucleotide 2′-methoxy modification. Agomirs behave like mature endogenous miRNA and are used for gain-of-function studies. Antagomir is chemically-modified single-strand miRNA inhibitor: 2 phosphorothioates at the 5′ end, 4 phosphorothioates at the 3′ end, 3′ end cholesterol group, and full-length nucleotide 2′-methoxy modification. Also called as antimiRs or blockmirs, Antagomirs constitutively inhibit the activity of target miRNAs and are used for loss-of-function studies.
structure of microRNA Agomir Antagomir

Get Quote for Custom Synthetic MicroRNA Agomir/Antagomir using your sequences or Pre-designed MIRacle™ MicroRNA Agomir/Antagomir

miRNA Agomir/Antagomir vs miRNA Mimic/Inhibitor

AcceGen MIRacle™ miRNA Agomir is the advanced product of miRNA mimic, MIRacle™ miRNA Antagomir is the advanced product of miRNA Inhibitor. AcceGen MIRacle™ miRNA Agomir/Antagomir are one of most innovative research tools with superior specificity and potency for elucidating the role of microRNAs.

Compared to other common miRNA mimics and inhibitors, MIRacle™ Agomirs and Antagomirs have the higher affinity for cell membranes, so the amount of needed transfection reagent could be drastically reduced in the cell transfection experiment to reduce experimental variables and improve the cost-efficiency; MIRacle™ Agomirs and Antagomirs have the higher simulating and inhibiting effect, so these products are suitable for the animals in vivo RNAi experiment by injection, inhalation, or feeding; MIRacle™ Agomirs and Antagomirs are more resistant to degradation, so the interfering effect can last at least 1 week, even up to 5-6 weeks for long-term miRNA functional studies.

Superiority

AcceGen’s microRNA agomir/antagomir synthesis service has the following advantages:

  • Cover all human, mouse, and rat miRNAs listed in miRBase
  • Higher simulated/inhibitory effects in vivo and in vitro
  • More stable, easier to pass the cell membrane and tissue gap
  • Purified and ready-to transfect cells/be administered by injection, inhalation, feeding
  • Less reagent use amount with longer effect time
  • Short synthesis cycle and cost-efficiency
MIRacle™ MicroRNA Agomir/Antagomir

MIRacle™ MicroRNA Agomir/Antagomir

SpeciesHuman / Mouse / RatSize/Quantity2 OD, 4 OD, 50 OD (size by request, 1 OD corresponds to 33 ug)
Shipping InfoRoom TemperatureStorage-20°C / -80°C
Quality ControlHPLCLabelFAM, CY3, CY5, etc. (optional)
ComponentMIRacle™ Agomir and/or Antagomir (Product Form: Dry Powder)
Agomir N.C and/or Antagomir N.C (optional)
ApplicationFor research use only
SpeciesHuman / Mouse / Rat
Size/Quantity2 OD, 4 OD, 50 OD (size by request, 1 OD corresponds to 33 ug)
Shipping InfoRoom Temperature
Storage-20°C / -80°C
Quality ControlHPLC
LabelFAM, CY3, CY5, etc. (optional)
ComponentMIRacle™ Agomir and/or Antagomir (Product Form: Dry Powder) Agomir N.C and/or Antagomir N.C (optional)
ApplicationFor research use only

Success Cases of Agomir/Antagomir Synthesis

AcceGen success case: MIRacle™ mmu-miR-140-3p miRNA Agomir
AcceGen success case: MIRACLE™ HSA-MIR-134-3P MIRNA ANTAGOMIR
AcceGen success case: MIRACLE™ HSA-MIR-134-5P MIRNA ANTAGOMIR
AcceGen success case: MIRACLE™ HSA-MIR-196b-5p MIRNA ANTAGOMIR
AcceGen success case: MIRACLE™ mmu-MIR-1a-3p MIRNA ANTAGOMIR
AcceGen success case: MIRACLE™ HSA-MIR-155-5P MIRNA ANTAGOMIR
AcceGen success case: MIRacle™ mmu-miR-140-3p miRNA Agomir
AcceGen success case: MIRACLE™ HSA-MIR-134-3P MIRNA ANTAGOMIR
AcceGen success case: MIRACLE™ HSA-MIR-134-5P MIRNA ANTAGOMIR
AcceGen success case: MIRACLE™ HSA-MIR-196b-5p MIRNA ANTAGOMIR
AcceGen success case: MIRACLE™ mmu-MIR-1a-3p MIRNA ANTAGOMIR
AcceGen success case: MIRACLE™ HSA-MIR-155-5P MIRNA ANTAGOMIR

Need a quote for MIRacle™ Agomir/Antagomir to improve your research project success or a consultation to discuss your project with our MicroRNA experts? CONTACT US NOW! We have significant bulk order discounts.

Applications of miRNA Agomir/Antagomir

miRNA Agomir Antagomir work drawingMicroRNA imperfect pair with target messenger RNAs (mRNAs) of protein-coding genes and regulate the transcription or post-transcription of their expression to involve in crucial biological processes, including development, differentiation, apoptosis, and proliferation. AcceGen MIRacle™ miRNA Agomir and Antagomir can be efficiently enriched in target cells to achieve specific and stable interference, so Agomir/Antagomir transfection into cells and injection in animals are the two main research applications. MIRacle™ miRNA Agomir/Antagomir are for research use only, however, these advanced products still have practical significance for the research of clinical trials, cancer treatments, and gene therapies, since it is speculated that miRNAs regulate one-third of human genes.

MIRacle™ miRNA Agomir/Antagomir Transfection Protocol

Specific microRNA Agomir/Antagomir is introduced into the cells which express the corresponding microRNA, and then it simulates the effect of microRNA to simulate/inhibit the effect of microRNA. Or the specific microRNA Agomir/Antagomir can be used to combine with the double luciferase reporter system/suppress the expression of a reporter vector, to verify the regulatory relationship between the miRNA and the target gene.

Transfection efficiency is different for different cell lines or different transfection reagents. The optimal transfection conditions still need to be determined by experiments. We suggest that the concentration range of miRNA Agomir is 1-100 nM and miRNA Antagomir is 15-100nM.

Recommended Agomir Transfection Procedure

96 well plate24 well plate12 well plate6 well plate
Transfection reagent0.2-0.6 μL0.5-2 μL1-3 μL2-5 μL
MicroRNA Agomir3 pmol15 pmol30 pmol75 pmol
Cell density6,000 cells/well40,000 cells/well80,000 cells/well200,000 cells/well
Final volume per well0.1 mL0.5 mL1.0 mL2.5 mL

Recommended Antagomir Transfection Procedure

96 well plate24 well plate12 well plate
Transfection reagent0.2-0.5 μL0.5-1 μL0.5-2 μL
MicroRNA Antagomir3 pmol15 pmol30 pmol
Cell density6,000 cells/well40,000 cells/well80,000 cells/well
Final volume per well0.1 mL0.5 mL1.0 mL
  • The recommended amount of transfection reagent should be adjusted appropriately according to the reagent you ordered;
  • The added amount shown is the final concentration of miRNA Agomir/Antagomir at 30 nM. It could be optimized according to the difference in the amount of maximum miRNA Agomir/Antagomir activity in different cell types;
  • Cell density is only a recommended value. There are certain changes in different cell lines, mainly depending on the size and growth of the cell. We recommend 30-70% cell fusion in general.

MIRacle™ miRNA Agomir/Antagomir in vivo Experiment Guideline

Through local injection or tail vein injection in animals, miRNA Agomir/Antagomir plays a role in local or systemic high expression of target miRNA. The dose of Agomir/Antagomir varies greatly with the way of administration. The dosage regimen is referred to as follows:

Recommended dosage per dose: Agomir/Antagomir 5-80μg/g body weight;

Dosage per mouse (body weight 15-20g): Agomir/Antagomir 200nmol (Generally need 3 injections).

Recommended Agomir/Antagomir Administration Routes

Intravenous administrationsuitable for heart, liver, kidney, lung, tumor tissues, and other tissues with rich blood flow6-week-old mouse, 45-150 μg, continuous injection for 3 days, test- 24 hours after the last injection
Respiratory administrationsuitable for the respiratory systemN/A
Intraperitoneal administrationsuitable for internal organs of the abdominal cavity and pelvis, pancreas, spleen, kidney, ovary, etc.N/A
Intracranial administrationsuitable for central nervous system researchN/A
Local administrationsuitable for sites difficult to reach with systemic administration, such as epidermis, subcutaneous (tumor), uterine cavity, etc.Xenograft nude mouse model, tumor Intra-injection, multi-point injection of 15μg, continuous 2-4 weeks, 2 times a week, 2-4 weeks after the first injection
Custom SynthesisPre-designed DatabaseTechnical Support
Order custom synthetic miRNA Agomir/AntagomirSearch in MIRacle™ miRNA Agomir & Antagomir DatabaseAny questions on miRNA Agomir/Antagomir, please contact us at info@accegen.com

Related MicroRNA Analyses

  • miRNA target prediction: to predict identified miRNA or your own sequences for miRNA target prediction and functional annotations in miRDB.
  • Analysis of miRNA expression level: to analyze the regulation level of miRNA, miRNA Agomir/Antagomir is transfected into cells for functional verification via Northern blotting and real-time quantitative fluorescent PCR.
  • Analysis of target gene expression level: in both miRNA Agomir/Antagomir transfected cells and negative control cells, real-time quantitative fluorescent PCR, and Western blotting is used to detect the expression level of the target gene and verify the regulatory relationship between miRNA and target gene.
  • Validation analysis of miRNA 3’UTR target site: by constructing one or more predicted miRNA 3’UTR binding target sites to the reporter plasmid, and co-transfecting miRNA Agomir/Antagomir with the reporter plasmid, to directly test the relationship between miRNA Agomir/Antagomir and miRNA predicted binding site.

We suggest that miRNA Agomir/Antagomir negative control is used as a reference in the experiment. The concentration and transfection conditions of the negative control are the same as those of the experimental group. Agomir N.C and Antagomir N.C can be optionally added with the synthetic miRNA Agomir/Antagomir products in your order.

MicroRNA Agomir/Antagomir Documents Download

To start your project faster, use the Standard Nomenclature to search below or browse our MicroRNA Agomir/Antagomir Database here. AcceGen provides over 5,000 pre-designed Human miRNA (hsa-miR) Agomir/Antagomir, Mouse miRNA (mmu-miR) Agomir/Antagomir and Rat miRNA (rno-miR) Agomir/Antagomir for worldwide MicroRNA research and diagnostics tools.

Species

However, if you can’t find your required product in our catalog, we are glad to provide custom synthetic MicroRNA Agomir/Antagomir to meet your specific needs. For more information about our MicroRNA Agomir/Antagomir Synthesis Service, please fill and submit the below Online Inquiry form, call us at 1-862-686-2696, or send an email to info@accegen.com. Your questions and requests will be answered by expert staff in AcceGen within 24 hours.
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