Home > Products > Primary Cells > Human Primary Cells > Nervous System Primary Cells >
Human Orbital Fibroblasts | ||||
|---|---|---|---|---|
| Product Name | Human Orbital Fibroblasts | |||
| Price | Get Quote | |||
| Cat.No | ABC-TC077G | Species | Human | |
| Cell Type | Fibroblast | |||
| Growth Mode | Adherent | |||
| Size/Quantity | 1 vial | Biosafety Level | 1 | |
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description | Human Orbital Fibroblasts (HOFs) from AcceGen are isolated from human orbital tissue. HOFs are cryopreserved at passage one and delivered frozen. Each vial contains over 0.5×106 cells in 1 mL volume. HOFs are characterized by their spindle morphology and immunofluorescence with antibody specific to vimentin. HOFs are guaranteed to further expand for more than 5 population doublings under the conditions provided by AcceGen. | |||
| Disease | Normal | |||
| Source Organ | Orbital Tissue | |||
| Quality Control | All cells test negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi. | |||
| Passage Number | 1 | |||
| Recommended Medium And Supplement | ABM-TM077G Human Orbital Fibroblasts Medium Kit | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | For research use only | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Questionsthree | What are the main applications of human orbital fibroblasts in scientific research? | |||
| Product Type | Orbital Cells | |||
| Product Image |  | |||
Frequently Asked Questions
What are human orbital fibroblasts, and what function do they serve?
Human orbital fibroblasts are specialized cells found in the connective tissue of the orbit. They help maintain the structural integrity of the orbit, produce extracellular matrix components, and contribute to tissue repair and remodeling. They are also involved in various physiological and pathological processes, including inflammation and fibrosis.
How are human orbital fibroblasts typically obtained and cultured for research purposes?
Human orbital fibroblasts are usually obtained from biopsy samples of orbital tissue. For culturing, these cells are grown in specialized media that support their growth and maintain their functionality. The media often contain nutrients, growth factors, and supplements to mimic the natural environment of orbital fibroblasts.
What are the main applications of human orbital fibroblasts in scientific research?
Human orbital fibroblasts are used to study conditions like thyroid eye disease (Graves’ orbitopathy), orbital inflammation, and fibrosis. They are important for developing and testing new treatments for these conditions and for understanding the mechanisms of tissue remodeling and scarring in the orbital region.
What are the key characteristics when culturing human orbital fibroblasts?
Key characteristics include their spindle-shaped morphology, proliferation rate, and expression of specific markers like vimentin and fibronectin. They should show healthy growth patterns, proper cell-to-cell interactions, and responsiveness to changes in media composition or the introduction of pharmacological agents.
What are the main challenges in culturing and experimenting with human orbital fibroblasts?
Challenges include maintaining their viability and functionality in vitro, replicating the complex natural environment, and preventing contamination. Variability between primary fibroblast cultures from different donors can affect experimental consistency. Another challenge is the potential for fibroblasts to become myofibroblasts under certain conditions, which can change their behavior and affect experimental results.
- ONLINE INQUIRY
- PRODUCT REVIEWS
Fill out a request in the form below and we’ll get back to you within 24 hours with a quote.
