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Happy New Year to everyone!
2020 is a tough year. Thanks for all the scientists and researchers who devote to biotech industry. Without your endeavor, the COVID-19 Vaccine wouldn’t be developed so fast.
For the moment, COVID-19 might be the most urgent problem to be overcome. The variant of the coronavirus has been reported. It was first identified in the United Kingdom, and then found in many other countries. Virus mutates all the time. There is no evidence that the new variants are more dangerous. But the new variant has been proved to spread faster, and there is potential catastrophe if this situation continues. Therefore, research on 2019-nCoV is still the most important task at the moment. Although the virus mutates constantly, the most suitable cell line for studying the virus is very fixed. Here we list the most related products to 2019-nCoV research for your information.
AcceGen is committed to supplying the best products and services in the field of life science to enhance the overall quality of life. And we are still expanding our cell repository. Fill in the online inquiry form or contact our customer team directly to inquire about the products or services you need. We will do our best to meet your specific research needs.
● AcceGen 2019-nCoV Research Related Products
2019-nCoV belongs to the β genus that shows a shape in the polymorphic, the virus is covered with fatty membrane, with its diameter in 60-220nm. The 2019-nCoV seems closely related with the severe acute respiratory syndrome coronavirus (SARS-CoV) that outbroke in 2002 because they show 76% in amino acid identity. And the mediate entry experiment shows that 2019-nCoV and SARS-CoV are capable of entering a similar spectrum of cell lines.
According to the result of the SARS-CoV invasion test, researchers have found some cells susceptible to 2019-nCoV, includes human cell lines 293T, Huh-7, Caco-2, and the animal cell lines Vero, MDCKII. When isolated and cultured in vitro, the 2019-nCoV can be found in human respiratory epithelial cells in about 96 hours. However, it takes about 6 days for the virus to be found if isolated and cultured in Vero E6 and Huh-7 cell lines.
The host cell factors ACE2 and TMPRSS2 play important roles in transfection. The receptor ACE2 provides an entry, and the host cell protease TMPRSS2 works as a primer that induces the S protein binding to a cellular receptor. And the effect of TMPRSS2 inhibitor is proved on nCoV-2019 infection experiments in cell lines. The interaction between S protein and ACE2 provides a solid foundation for drug and vaccine design, and the level of related cells may play an important role in testing and promotion.
These mechanisms are discovered and verified by cell experiments. 293T, Vero (TMPRSS2-) and Caco-2 (TMPRSS2+) are chosen as targets. Camostat mesylatel, the clinically proven serine protease inhibitor, could work as a TMPRSS2 inhibitor that partially blocked 2019-nCoV entry into Caco-2 and genetically modified VERO-TMPRSS2 cells. However, the inhibition is less efficient in the 293T and Vero cell lines.
Like other viruses, 2019-nCoV utilize animals as the primary host. The human airway epithelium (HAE) represents the entry port of many human respiratory viruses, including the coronavirus. 2019-nCoV attack and inflame respiratory epithelial cells, inducing a severe respiratory illness and pneumonia. When isolated and cultured in vitro, the 2019-nCoV can be found in human respiratory epithelial cells in about 96 hours. It is much sooner than cell lines which could take 6 days for the virus to be found in cultured Vero E6 and Huh-7 cell lines. Thus, we recommend respiratory epithelial cells (like Human Bronchial Epithelial Cells) that could soon be infected by 2019-nCoV. Unlike SARS-CoV or MERS-CoV, the 2019-nCoV grows better in primary human airway epithelial cells than in standard tissue-culture cells.
For inquiries about the products, please contact email@example.com. We wish our resources could support related research to move forward.
To further support your research, AcceGen R&D team has also posted serval 2019-nCoV articles on our website, which might provide insights into the viral transmission and vaccine development.
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