C57BL/6 Mouse Dermal Fibroblasts

Description

C57BL/6 Mouse Dermal Fibroblasts are primary cells isolated from the skin of mice. These cells reside exclusively within the dermis layer of the skin. Following primary culture, these cells are cryopreserved. These mouse fibroblasts play crucial roles in extracellular matrix synthesis, wound contraction, and immune modulation through cytokine signaling. Studies have demonstrated their implication in pathological fibrosis, scleroderma-like connective tissue disorders, and diabetic chronic wound healing impairment due to hypercontractility and ECM dysregulation, driving therapeutic development. The cells exhibit a characteristic spindle-shaped morphology and are validated by fibroblast-specific protein 1 (FSP1/S100A4). For optimal performance, repeated freezing and thawing should be avoided during culture. The cells undergo rigorous screening and isolation procedures, and are rigorously tested to ensure they are free of contamination from Mycoplasma, Fungi, Yeast, and Bacteria.