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Primary Cells

Human Alveolar Macrophages

  • For research use only

Cat No.

ABC-H0034X
Product Type

Pulmonary Cells
Cell Type

Macrophage
Species

Human
Growth Conditions

37 ℃, 5% CO2
Source Organ

Alveolar
Disease

Normal/Non-Small Cell Lung Cancer
Storage

Liquid Nitrogen

Human Alveolar Macrophages, non-proliferative phagocytes from lung lavage, mediate barrier defense, inflammation, and pathogen clearance in research.

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  • Human Alveolar Macrophages
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Home | Primary Cells | Human Primary Cells | Respiratory System Primary Cells | Human Alveolar Macrophages

Description

Human Alveolar Macrophages are terminally differentiated, non-proliferative primary cells typically isolated from bronchoalveolar lavage fluid of healthy donors or patients with lung diseases. They reside within the alveolar spaces and at the air–liquid interface of the alveoli. These mononuclear phagocytes exhibit an irregular, flattened morphology and adhere to culture vessels, often requiring GM-CSF (and, in vitro, M-CSF) for in viability maintenance. They secrete cytokines and chemokines such as TNF-α and IL-1β to initiate inflammation and recruit neutrophils, and phagocytose apoptotic or necrotic cells. Alveolar macrophages express markers like CD68, CD11c, and CD206. Their functional responses are mediated through receptors like TLR4, activating NF-κB signaling. Phenotypic shifts occur in disease states—e. g., an M2-like profile in asthma. These pulmonary alveolar macrophages provide a critical model to study alveolar immune responses, allowing researchers to observe macrophages cells at work and compare human primary macrophages with murine MH-S cells in preclinical respiratory research.

Species

Human
Cat.No

ABC-H0034X
Product Category

Primary Cells
Size/Quantity

1 vial
Cell Type

Macrophage
Growth Mode

Adherent
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Alveolar
Disease

Normal/Non-Small Cell Lung Cancer
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Pulmonary Cells
Key Features

– Backed by AcceGen advanced technology

– Cryopreserved for highest viability and plating efficiency

– Quality-guaranteed for accurate results

– Timely technical support for best experimental outcomes
Quality Control

All cell lots test negative for HIV-1, HBV, HCV, mycoplasma, yeast, fungi, and other pathogens.

Application

  • Human Alveolar Macrophages can be used as an in vitro model to study lung immune mechanisms, pathogen-host interactions and intercellular communication, which helps to gain a deeper understanding of the initiation and regulation of lung innate immunity and adaptive immunity. These cells can also be used to explore the pathogenesis of lung diseases (such as pneumonia, lung cancer, and chronic obstructive pulmonary disease) and further understand the functional changes of macrophages in these diseases.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Laurent P, Yang C, Rendeiro AF, et al. Sensing of SARS-CoV-2 by pDCs and their subsequent production of IFN-I contribute to macrophage-induced cytokine storm during COVID-19. Sci Immunol. 2022;7(75):eadd4906. doi:10.1126/sciimmunol.add4906

Frequently Asked Questions

  • What are Human Alveolar Macrophages?

    Human Alveolar Macrophages are specialized immune cells derived from the lung’s alveolar spaces. They play a critical role in the innate immune defense system by engulfing and digesting pathogens, cellular debris, and foreign particles inhaled into the lungs. These cells are crucial for maintaining pulmonary homeostasis and responding to respiratory infections.

  • What is the process for isolating Human Alveolar Macrophages?

    Human Alveolar Macrophages are typically isolated from bronchoalveolar lavage fluid obtained during bronchoscopy procedures. The lavage fluid is centrifuged to separate the cells, which are then purified using techniques such as adhesion to plastic surfaces or gradient centrifugation to enrich the macrophage population. These cells can be cultured for research purposes, providing a model to study lung immunity and disease mechanisms.

  • What are the main functions of Human Alveolar Macrophages?

    The main functions of Human Alveolar Macrophages include:
    – Phagocytosis: Engulfing and digesting bacteria, viruses, and other foreign particles.
    – Cytokine Production: Secreting signaling molecules that modulate the immune response, including pro-inflammatory and anti-inflammatory cytokines.
    – Antigen Presentation: Processing and presenting antigens to T cells to initiate adaptive immune responses.
    – Tissue Repair and Remodeling: Contributing to the repair of damaged lung tissue and maintaining tissue homeostasis.

  • What are the primary applications of Human Alveolar Macrophages in research?

    Human Alveolar Macrophages are used in a variety of research applications, including:
    – Studying Pulmonary Diseases: Investigating the pathogenesis of diseases such as asthma, chronic obstructive pulmonary disease (COPD), tuberculosis, and lung cancer.
    – Drug Testing and Development: Evaluating the efficacy and safety of new therapeutic agents targeting respiratory diseases.
    – Immune Response Analysis: Understanding the immune mechanisms and responses to pathogens, allergens, and pollutants.
    – Inflammation Research: Exploring the molecular pathways involved in lung inflammation and identifying potential targets for anti-inflammatory therapies.

  • Do alveolar macrophages proliferate in vitro？

    Alveolar macrophages have limited proliferative capacity in vitro. These terminally differentiated cells primarily function to defend and clear pathogens and foreign substances inhaled into the lungs. While limited proliferation can occur under specific conditions with certain growth factors or specialized culture media, they generally do not proliferate significantly. Researchers typically isolate fresh macrophages from lung tissue for experiments to ensure their functionality and activity.

  • What are alveolar macrophages?

    Alveolar macrophages are specialized macrophages residing in the alveolar space of the lungs.

  • Where are alveolar macrophages found?

    Alveolar macrophages are found on the luminal surface of the alveoli, within the alveolar air spaces of the lung, where they directly interact with inhaled particles and pathogens.

  • What is the function of alveolar macrophages?

    Alveolar macrophages mediate phagocytosis of pathogens, particulates, and cellular debris. They also regulate pulmonary immune responses and maintain alveolar homeostasis through cytokine and chemokine signaling.

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