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Human Dopaminergic Neuron Cells

ImageAcceGen cells & cell lines
Product Name

Human Dopaminergic Neuron Cells

Product Code

HDaNCs

Cat.No

ABC-TC5463

Product Type

Nervous Cells

Species

Human

Biosafety Level

1

Size/Quantity

1 vial

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Human dopaminergic neuronal cells are isolated from fetal brain tissue obtained from agencies authorized to procure and distribute tissues for research. Dopaminergic neuronal cells are selected in tyrosine free medium supplemented with a mixture of growth factors. Actively growing population of cells are tested for tyrosine hydroxylase (TH) expression by immunocytochemistry. HDaNCs cells are provided at more than 5 x 10[5] cells/vial @ Passage 1 with more than 70% TH positive. HDaNCs are grown in HDaNCs medium. The cells are grown in regular tissue culture dishes and subcultured at a split ratio of 1 to 2 at confluence. We recommend not to use the cells beyond 4 passages. There may be a significant decrease in the number of tyrosine hydroxylase positive cells after 4 passages as they are not maintained in selective medium to allow the growth of only cells expressing tyrosine hydroxylase.

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Citation Guide

If the product is used for publication, it should be cited in the publication as: "AcceGen Cat.# XXX-0000". In addition, click here to submit your paper’s PubMed ID (PMID) to get a $100 coupon.

Application

For research use only

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