Human Eosinophilic Esophagitis Peripheral Blood Mononuclear Cells

Description

Human Eosinophilic Esophagitis Peripheral Blood Mononuclear Cells (EoE PBMCs) are immune cells isolated from peripheral blood of patients clinically diagnosed with active eosinophilic esophagitis (EoE), typically defined by ≥ 15 eosinophils per high-power field in esophageal biopsies. These cells show elevated activation of CD4⁺ T lymphocytes and myeloid subsets, marked by increased surface expression of CD38 and Programmed Cell Death Protein 1 (PD‑1) compared to patients in remission, gastroesophageal reflux disease (GERD), and healthy controls. EoE PBMCs interact with primary esophageal epithelial cells in co-culture models to upregulate checkpoint molecules such as PD‑1, indicating direct cell–cell immune-epithelial crosstalk. They serve as a valuable in vitro system to investigate T helper 2 (Th2)-driven inflammation, T-cell exhaustion, and novel immunotherapeutic targets.