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Immortalized Mouse Dendritic Cell Line

Product Name

Immortalized Mouse Dendritic Cell Line

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Cat.No

ABI-TC329D

Species

Mouse

Size/Quantity

1 vial

Biosafety Level

2

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

As messengers bridging the innate and adaptive immune system, the conventional tissue-resident DC (cDC) acts as sentinels in secondary lymphoid organs and other tissues for antigen capture and presentation. The Wild-type MutuDC1940 cell line is an Immortalised splenic CD8α+ subset (CD11chigh,B220-,DEC205+,CD24high,CD11b-) of conventional dendritic cells derived from the CD11c:SV40LgT transgenic mice. The MutuDC1940 cell line retains response to TLR ligands such as CpG (TLR9-L) and PolyIC (TLR3-L) and to a lesser extent LPS (TLR4-L) stimulation by up-regulation of co-stimulatory molecules CD40, CD80 and CD86. In addition to responding to PAMP stimulation and producing Th1 cytokines such as IL-12, these cells are also capable of presenting antigen in the context of both MHC-I and MHC-II, including direct antigen presentation and cross-presentation of cell-associated antigens.

Disease

Normal

Source Organ

Lymphoid

Quality Control

1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA, 4) Response to PAMP stimulation evaluated by functional assays.

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Citation Guide

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Application

For research use only

Morphology

Small aggregates

Immortalization Method

Isolated from C57BL/6 transgenic mice carrying SV40 Large T oncogene

Growth Conditions

37 ℃, 5% CO2

Cell Type

DC Cell

Growth Mode

Adherent

Product Type

Immortalized Cell Line

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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