| Species |
Mouse
|
| Cat.No |
ABI-TC329D
|
| Quality Control |
1) Direct antigen presentation and cross-antigen presentation were evaluated by the MHC-I (SIINFEKL/OT-I ) and MHC-II (OVA323-339/OT-II) restricted systems; 3) proteome profile and surface markers assessed by RT-PCR; RT-PCR; 3) IL-12 cytokine secretion analyzed using ELISA, 4) Response to PAMP stimulation evaluated by functional assays.
|
| Product Category |
Immortalized Cell Lines |
| Size/Quantity |
1 vial
|
| Cell Type |
DC Cell
|
| Shipping Info |
Dry Ice
|
| Growth Conditions |
37 ℃, 5% CO2
|
| Source Organ |
Lymphoid
|
| Disease |
Normal
|
| Biosafety Level |
2
|
| Storage |
Liquid Nitrogen
|
| Product Type |
Immortalized Cell Line
|
| Immortalization Method |
Immortalized Mouse Dendritic Cell Line was immortalized by transducing C57BL/6 mouse bone marrow isolates with retroviral vectors expressing GM-CSF, myc, and raf oncogenes.
|
Description
An immortalized mouse dendritic cell line refers to a type of cell line derived from mouse dendritic cells that have been modified to proliferate indefinitely in cell culture. Dendritic cells are a specialized type of immune cell responsible for presenting antigens to T cells, thereby initiating and regulating immune responses. Immortalized mouse dendritic cell lines are valuable tools in immunological research, as they provide a consistent and reproducible source of dendritic cells for studying various aspects of the immune system, including antigen presentation, immune activation, and tolerance mechanisms.
These cell lines are often generated through the introduction of genetic modifications or viral transformation to overcome the limited lifespan of primary dendritic cells in culture. Alternatively, dendritic cells can be fused with myeloma cells to create hybridomas that exhibit both the antigen-presenting capabilities of dendritic cells and the immortalized growth properties of myeloma cells.
Immortalized mouse dendritic cell lines offer several advantages for research purposes. They provide a consistent and homogeneous population of dendritic cells that can be easily expanded in culture, allowing for large-scale experiments and high-throughput screening assays. These cell lines also retain many of the functional characteristics of primary dendritic cells, including the ability to respond to microbial stimuli, present antigens to T cells, and regulate immune responses.
Why choose Immortalized Mouse Dendritic Cell Line from AcceGen?
Our stringent quality control measures guarantee the exceptional functionality of these dendritic cells:
Antigen Presentation Evaluation
Rigorous assessments of direct antigen presentation and cross-antigen presentation in MHC-I (SIINFEKL/OT-I) and MHC-II (OVA323-339/OT-II) restricted systems, ensuring accurate and efficient antigen processing.
Proteome Profiling and Surface Marker Assessment
Utilizing RT-PCR, we thoroughly examine the proteome profile and surface markers, providing insights into the cell line’s characteristics and ensuring reliability.
IL-12 Cytokine Secretion Analysis
Employing ELISA, we analyze IL-12 cytokine secretion, offering valuable information about the cell line’s immune response and functionality.
PAMP Stimulation Responsiveness
Functional assays evaluate the cell line’s response to PAMP stimulation, ensuring its suitability for various experimental conditions.
