Immortalized Mouse Dendritic Cell Line

Description

Immortalized Mouse Dendritic Cells (Mouse DCs) are derived from murine bone marrow and transformed via retroviral transduction of v-raf and v-myc oncogenes to enable continuous proliferation while preserving key dendritic cell functions. The cells retain key functional attributes of dendritic cell lines, providing a stable and renewable in vitromodel for immunological research, where they are often used as a counterpart to primary dendritic cells or other established lines like DC2.4. These cells display characteristic dendritic morphology with cytoplasmic projections and grow adherently or semi-adherently in vitro. They express canonical dendritic markers, including MHC class II, CD11c, CD80, and CD86. Functionally, they retain antigen-presenting capabilities, secrete cytokines such as IL-12 and TNF-α, and effectively stimulate T cells. Offering a scalable and reproducible alternative to primary dendritic cells, they are widely used in studies of antigen processing, T-cell activation, tumor immunology, vaccine development, and autoimmune disease mechanisms.