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| Species | Mouse |
| Cat.No | ABI-TC4178 |
| Product Category | Immortalized Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Macrophage |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Alveolus |
| Disease | Normal |
| Storage | Liquid Nitrogen |
| Product Type | Immortalized Cell Lines |
The MH-S cell line is a continuous culture of murine alveolar macrophages (AM), originally derived from bronchoalveolar lavage cells of Balb/cJ mice via transformation with simian virus 40 (SV40). These cells are characterized by their adhesion properties, with a doubling time of approximately 48 hours. MH-S cells maintain typical macrophage morphology, with over 98% esterase-positive cells, while being negative for peroxidase. They lack contact inhibition, and is sensitive to trypsin treatment. MH-S express intracellular T-antigen and manifest DNA synthesis through the incorporation of 3H-thymidine. They further express cell surface markers, including Ia and Mac-1 antigens, and exhibit Fc receptor positivity. Notably, MH-S cells maintain a key functional property of freshly isolated alveolar macrophages: the capacity to suppress the in vitro plaque-forming cell (PFC) response in a dose-dependent manner when co-cultured with splenic lymphocytes.
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The MH-S cell line is a valuable tool in immunological research, particularly in studies involving alveolar macrophage function. Its consistent phenotype and functional properties make it ideal for experiments requiring homogeneous macrophage populations. MH-S cells serve as a robust model for elucidating the role of alveolar macrophages in lung immunity and pathology, contributing to our understanding of respiratory diseases and host immune responses in pulmonary context.
The MH-S cell line is a macrophage, alveolar cell line, originally derived from the bronchoalveolar lavage of BALB/cJ mice and transformed with simian virus 40 (SV40).
These cells express cell surface Ia and Mac-1 antigens, are over 98% esterase-positive, negative for peroxidase, and exhibit Fc receptor positivity which supports their phagocytic activity.
MH-S cells are notable for their macrophage morphology, adherent and suspension growth properties, expression of intracellular T-antigen, and the ability to manifest DNA synthesis. They also display significant adhesion properties, lack of contact inhibition, and sensitivity to trypsin.
MH-S cells should be cultured in RPMI 1640 medium supplemented with 2mM Glutamine, 0.05mM 2-Mercaptoethanol, and 10% Fetal Bovine Serum (FBS).
MH-S cells are extensively used in immunology research, particularly for studies focusing on the role of alveolar macrophages in immune responses and lung pathology. They are crucial for experiments requiring homogeneous populations of alveolar macrophages.
