For research use only
| Cat No. | ABI-TC4178 |
| Product Type | Immortalized Cell Lines |
| Cell Type | Macrophage |
| Species | Mouse |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Alveolus |
| Disease | Normal |
| Storage | Liquid Nitrogen |
Apply MH-S alveolar macrophage line for pulmonary immunology, respiratory infection modeling, and macrophage biology research in murine systems.
The MH-S cell line is a continuous culture of murine alveolar macrophages (AM), originally derived from bronchoalveolar lavage cells of Balb/cJ mice via transformation with simian virus 40 (SV40). These cells grow as a mixed adherent and suspension population demonstrating typical macrophage morphology, express intracellular SV40 T antigen, and lack contact inhibition. Over 98 % of the cells are esterase-positive, peroxidase-negative, and they express Fc receptors, Mac‑1 (CD11b), and MHC II antigens. MH-S express intracellular T-antigen and manifest DNA synthesis through the incorporation of 3H-thymidine. The line shows robust innate immune activity, including phagocytosis of antibody-coated particles and constitutive IL‑1 secretion, which increases upon LPS stimulation. MH‑S also suppresses plaque-forming cell responses in co‑cultures with splenocytes, similar to primary alveolar macrophages. These characteristics make MH‑S a reliable model of alveolar macrophage biology with defined functional and immunophenotypic profiles.
| Product Code | MH-S, MH-S Cells, MH-S Cell Line, Mouse Alveolar Macrophage Cell Line MH-S |
| Species | Mouse |
| Cat.No | ABI-TC4178 |
| Product Category | Immortalized Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Macrophage |
| Growth Mode | Adherent |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Alveolus |
| Disease | Normal |
| Storage | Liquid Nitrogen |
| Product Type | Immortalized Cell Lines |
The MH-S cell line is a valuable tool in immunological research, particularly in studies involving alveolar macrophage function. Its consistent phenotype and functional properties make it ideal for experiments requiring homogeneous macrophage populations. MH-S cells serve as a robust model for elucidating the role of alveolar macrophages in lung immunity and pathology, contributing to our understanding of respiratory diseases and host immune responses in pulmonary context.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
The MH-S cell line is a macrophage, alveolar cell line, originally derived from the bronchoalveolar lavage of BALB/cJ mice and transformed with simian virus 40 (SV40).
These cells express cell surface Ia and Mac-1 antigens, are over 98% esterase-positive, negative for peroxidase, and exhibit Fc receptor positivity which supports their phagocytic activity.
MH-S cells are notable for their macrophage morphology, adherent and suspension growth properties, expression of intracellular T-antigen, and the ability to manifest DNA synthesis. They also display significant adhesion properties, lack of contact inhibition, and sensitivity to trypsin.
MH-S cells should be cultured in RPMI 1640 medium supplemented with 2mM Glutamine, 0.05mM 2-Mercaptoethanol, and 10% Fetal Bovine Serum (FBS).
MH-S cells are extensively used in immunology research, particularly for studies focusing on the role of alveolar macrophages in immune responses and lung pathology. They are crucial for experiments requiring homogeneous populations of alveolar macrophages.
