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Hot Products
- In-Stock Tumor Cell Lines
- Human Orbital Fibroblasts
- Human Microglia
- Human Pulmonary Alveolar Epithelial Cells
- Human Colonic Fibroblasts
- Human Type II Alveolar Epithelial Cells
- Human Valvular Interstitial Cells
- Human Thyroid Epithelial Cells
- C57BL/6 Mouse Dermal Fibroblasts
- Human Alveolar Macrophages
- Human Dermal Fibroblasts, Adult
- Human Lung Fibroblasts, Adult
- Human Retinal Muller Cells
- Human Articular Chondrocytes
- Human Retinal Pigment Epithelial Cells
- Human Pancreatic Islets of Langerhans Cells
- Human Kidney Podocyte Cells
- Human Renal Proximal Tubule Cells
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What is the origin of HighQC™ GFP Expressing Human Induced Pluripotent Stem Cells, and how are they constructed?
This cell line was derived from fibroblasts that were reprogrammed into iPSCs. The fibroblasts were reprogrammed using retroviral vectors carrying the OCT4, SOX2, KLF4, and MYC gene sequences. After obtaining the iPSCs, the cells were infected with GFP-expressing lentivirus or RFP-expressing lentivirus, resulting in iPSC cell lines labeled with GFP or RFP.
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What is the doubling time and passage limit for HighQC™ Human Embryonic Stem Cells?
Doubling time is approximately 24-36 hours, depending on culture conditions. To maintain their pluripotency, it is recommended to keep the passage number below 20.
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How are HighQC™ Human Hair Follicle Stem Cell isolated?
These cells are isolated using a combination of collagenase and neutral protease digestion.
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What method was used to immortalize Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells?
These cells are typically immortalized using SV40. Alternatively, hTERT can be used upon request. However, all immortalized stem cells may lose some differentiation potential.
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What is the biosafety level of the cells?
The biosafety level of these cells is BSL-2.
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What is a stem cell?
Stem Cells, also calls Progenitor Cells, are a kind of self-replicating pluripotent cells, which can differentiate into multiple functional cells under certain conditions. Given this characteristic, some diseases can be cured by transplanting stem cells or related derivatives into patients to replace damaged cells.
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Can I use your stem cells for curing disease?
Unfortunately, stem cells provided by AcceGen are only for research use. We would suggest you to seek help from professional hospital or therapeutic center.
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How many times can stem cells be passaged?
Times would vary from different types of stem cells. For example, pluripotent stem cells which are derived from the inner cell mass of blastocysts or induced by transcription factors from somatic cells, hPSC can self-renew indefinitely. If you would like to know the exact passage times of your stem cells, please consult our customer time.
Theoretically, pluripotent stem cells have an infinite lifespan. Due to the culture condition restriction, the more time cells passaged, the higher risk of chromosomal differentiation or instability. The karyotype analysis is recommended every 10 passages.
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Can I expand or cryopreserve hPSC-derived cardiomyocytes?
We would not suggest you to expand or re-freeze hPSC-derived cardiomyocytes in any circumstances.
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What is the difference between embryonic stem cells, adult stem cells, and umbilical cord stem cells?
Embryonic Stem Cells are a type of cells isolated from early embryos or primitive gonads. They have the characteristics of infinite proliferation, self-renewal, and multi-directional differentiation. Whether in vitro or in vivo, ES cells can be induced to differentiate into almost all cell types in the body.
Adult Stem Cells are undifferentiated cells that exist in a differentiated tissue that can self-renew and specialize to form the cells that make up this type of tissue. Adult stem cells are found in various tissues and organs of the body
Umbilical cord stem cells are isolated from the umbilical cord of newborn infants and are less mature than adult stem cells. Umbilical cord stem cells are a type of somatic stem cell.
