Technical Support
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How can I determine whether Agomir/Antagomir has been successfully delivered into cells?
Cellular uptake can be directly observed using a fluorescently labeled version. Alternatively, successful delivery can be indirectly confirmed by detecting changes in the expression of downstream target genes.
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Is it necessary to include a negative control at the same time?
Yes. A negative control is essential to exclude the effects of transfection and non-specific responses, and it serves as a critical control group for evaluating the effects of Agomirs/Antagomirs.
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Do Agomirs/Antagomirs have high cytotoxicity to cells?
The oligonucleotides themselves generally exhibit low cytotoxicity; however, transfection reagents or high-dose treatments may induce cellular stress or reduced proliferation. It is recommended to include a negative control group in the experimental design.
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How long can Agomir/Antagomir be stored after reconstitution?
After reconstitution, the product should be aliquoted and stored at −20 °C to avoid repeated freeze–thaw cycles. It is generally stable for 3–6 months. Before use, ensure there are no signs of degradation.
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What is the difference between MicroRNA Agomir and Antagomir?
Agomirs are used to enhance the function of specific miRNAs, whereas antagomirs are designed to inhibit miRNA activity. Both are chemically modified and suitable for both in vitro and in vivo experiments.
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What is the minimum quantity you can provide for miRNA Agomir/Antagomir service?
The minimum quantity is 2 OD (1 OD corresponds to 33 μg). Please note we have significant bulk order discounts.
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What kinds of labels you can do for miRNA Agomir/Antagomir service?
We can usually label the miRNA Agomir/Antagomir with fluorescent tag, such as FAM, CY3, etc.
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How to convert the OD and nmol of RNA oligo?
microRNA agomirs are double-stranded, while microRNA antagomirs are single-stranded. The amount of materials are quantified in OD and both agomirs/antagomirs are synthesized to yield the same OD. In general, there is a simple calculation as follows.
Agomir: 1 OD duplex=2.5 nmol = 33µg; Antagomir: 1 OD simplex=5 nmol = 33µg.
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What should be provided for microRNA agomir/ antagomir synthesis?
To synthesize microRNA agomir/antagomir, you just need to provide the accession number of the mature microRNA and the quantity you need to synthesize.
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Can the negative control (NC) of agomir and antagomir be used universally? Does NC have to be used?
It’s not universal, because agomir is a double-stranded RNA and antagomir is a single-stranded RNA. It is recommended to use NC as a control group to exclude false positive results.
Click the picture or title to enter the corresponding page to browse AcceGen MicroRNA Agomir/Antagomir products:
– Human MicroRNA Agomir/Antagomir
– Mouse MicroRNA Agomir/Antagomir
– Rat MicroRNA Agomir/AntagomirFor more detailed or not mentioned technical questions, contact us at +1-862-248-1155 or inquiry@accegen.com, our customer service department is always here to support you.
