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Hot Products
- In-Stock Tumor Cell Lines
- Human Orbital Fibroblasts
- Human Microglia
- Human Pulmonary Alveolar Epithelial Cells
- Human Colonic Fibroblasts
- Human Type II Alveolar Epithelial Cells
- Human Valvular Interstitial Cells
- Human Thyroid Epithelial Cells
- C57BL/6 Mouse Dermal Fibroblasts
- Human Alveolar Macrophages
- Human Dermal Fibroblasts, Adult
- Human Lung Fibroblasts, Adult
- Human Retinal Muller Cells
- Human Articular Chondrocytes
- Human Retinal Pigment Epithelial Cells
- Human Pancreatic Islets of Langerhans Cells
- Human Kidney Podocyte Cells
- Human Renal Proximal Tubule Cells
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How does freezing and thawing immortalized cells differ from regular cells?
The process is basically the same. However, immortalized cells generally proliferate faster after thawing, and adherent cells usually reattach within 12–24 hours.
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Are immortalized cells equivalent to tumor cells?
Not exactly. Some tumor cells naturally possess immortal characteristics, whereas primary cells that have been immortalized through transfection generally retain a more primitive phenotype and function. They do not exhibit the high proliferation and invasiveness typical of tumor cells.
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Does immortalization affect cell phenotype?
It may, but the extent depends on the immortalization method and the cell type. For example, SV40 Large T or HPV E6/E7 can alter proliferation, cell cycle, or certain signaling pathways. In terminally differentiated cells like neurons, which are initially non-proliferative and morphologically specialized, the cells after transfection and selection may differ significantly from the original primary cells.
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Do immortalized cells exhibit batch-to-batch variations?
Yes, but usually to a lesser extent than primary cells. Factors that can influence variation include:
The immortalization method used (e.g., hTERT vs. SV40T)
Passage number and culture duration
Different donors
For large-scale experiments, it is recommended to purchase multiple vials from the same batch and use them consistently. -
What are immortalized cells?
Immortalized cells are cell lines that have bypassed the normal replicative limit and can be stably propagated long-term, either through genetic modifications (e.g., hTERT, SV40 Large T, HPV E6/E7) or spontaneous mutations.
