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Can iPSC cells be grown without a feeder layer? If a feeder layer is required are you using MEF cells?
iPSCs usually grow best when attached to other cells or an extracellular matrix and grow traditionally in cultures with feeder layers.
MEFs are fairly universally used. If using MEFs as a feeder layer, the MEF media must be removed before the iPS cells can be plated. The iPS cells can be cultured in MEF dishes conditioned with iPS medium. We recommend conditioning new feeder dishes prior to passaging iPS cells into them.
