FAQs

FAQ

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  • What is the passage limit and doubling time of Human Preadipocytes-Subcutaneous under standard culture conditions?

    Human Preadipocytes-Subcutaneous can be passaged up to 4 times, with a doubling time of approximately 24-48 hours. However, the specific conditions depend on experimental procedures, cell status, and culture conditions.

  • What is the source of Human Oral Epithelial Cells?

    AcceGen's Human Oral Epithelial Cells are mainly isolated from the gingival mucosa and buccal mucosa of human oral cavity.

  • What is the source of Human PB Red Blood Cells/Mature Erythrocytes?

    The peripheral blood is collected by leukapheresis from fully consented IRB approved donors who are tested negative for HIV, HBV, and HCV. A common anticoagulant acid-citrate-dextrose formula A (ACD-A) is used to prevent the blood from clotting during the leukapheresis process.

  • What is the passage limit for Human Lobar Bronchial Epithelial Cells?

    These cells are typically viable for 10-12 passages before cellular senescence.

  • What lymphocyte types are present in Human Spleen Cells?

    Human Spleen Cells contain T cells, B cells, and NK cells. Among them, T cells and B cells are present in higher proportions, while NK cells are less abundant.

  • Do Human Ovarian Surface Epithelial Cells require gelatin coating?

    These primary cells adhere poorly at the begining, so gelatin coating is recommended. In later passages, coating might be reduced or omitted.

  • What is the species information and coating concentration of collagen required for Human Microglia?

    The Type I collagen provided by AcceGen is purified from rat tail tendons. The recommended coating concentration is 0.5 μg/cm².

  • What is the passage limit and doubling time for Human Corneal Keratocytes?

    Human Corneal Keratocytes can be passaged 12-15 times, with a doubling time of approximately 24-36 hours. However, the specific conditions may vary depending on experimental procedures, cell status, and culture conditions.

  • How are Human Esophageal Epithelial Cells obtained?

    These cells are isolated from human esophageal tissue using trypsin digestion.

  • What quality control is performed for Human Mast Cells – Dermis?

    AcceGen evaluates the viability of mast cells by flow cytometry (>80%) and characterizes them using surface markers including CD117, FcεR1a, chymase, and tryptase. In addition, AcceGen tests the cells for common microbial contamination and blood-borne pathogens including HIV-1, HIV-2, Hepatitis B, and Hepatitis C.


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