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Product Code

HEK-293A; HEK293A; HEK 293A; QBI-HEK 293A; QBI-293A






1 vial

Biosafety Level


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Dry Ice


Liquid Nitrogen


The 293A cell line is a subclone derived from HEK293 cells and is characterized by its flat morphology. HEK293 cell was originally established from primary embryonal human kidney cells that had undergone transformation with sheared human adenovirus type 5 DNA. Notably, 293A cells express the genes encoded by the E1 region of adenovirus, which play a pivotal role in transactivating certain viral promoters. This unique feature enables 293A cells to produce exceptionally high levels of protein. Additionally, the expression of E1 genes in 293A cells allows them to complement E1-deleted recombinant adenoviral vectors, facilitating viral replication in research applications.


Why choose 293A from AcceGen?

The 293A cell line from AcceGen is characterized by its exceptional viability and quality, maintained through rigorous quality control measures. These cells are routinely monitored for sterility to ensure the absence of bacteria and fungi, and their mycoplasma-free status is confirmed using PCR analysis. Identity is verified through STR analysis, and the cells are incubated under optimal conditions before being cryopreserved using advanced techniques, ensuring the reliability and integrity of this valuable research tool.

Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).



The 293A cell line serves as a vital tool in various applications, primarily enabling the streamlined production, amplification, and titering of replication-incompetent adenovirus vectors. Its unique attributes make it an indispensable resource for researchers aiming to screen and develop adenoviral constructs with precision, ensuring the creation of high-quality viral preparations for a wide range of biomedical and molecular biology studies.

Growth Conditions

37 ℃, 5% CO2

Product Type

Human Kidney Cell Lines

Product Image

hek 293a cell lineAcceGen product hek 293a cell line

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