Tissue: kidney, embryonic; Transformed by: SV40 large T antigen; Original line: 293. Used for transfection assays and as packaging cells. The cell line can be transiently transfected and give extremely high levels of expression of AP fusion proteins. G418 resistant, neomicine resistant. The cell line is known also as 293tsA1609neo.
|Recommended Medium And Supplement|
DMEM + 20% FBS + 2mM L-Glutamine; Split subconfluent cultures (70% confluence)1: 4-1: 8 using trypsin/EDTA; 37°C, 5% CO2
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For research use only
Human Kidney Cell Lines
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