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AceSeq™ λ Phage DNA Kit |
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Product Name |
AceSeq™ λ Phage DNA Kit |
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Price | Get Quote | |||
Cat.No |
AG062Z |
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Size/Quantity |
Kit |
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Shipping Info |
Ice Pack |
Storage |
Room Temperature |
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Description |
AceSeq™ λ Phage DNA Kit is suitable for rapidly extracting DNA from λ phage lysate cultures. After centrifugation of λ phage lysate culture, the supernatant is first digested with RNase A/DNase I mixed enzyme to remove the residual host bacterial DNA/RNA, and the phage is collected by precipitation. The phage is cleaved by SDS, and the residual debris is removed by precipitation centrifugation. The λ phage DNA in the supernatant of the lysate is selectively adsorbed to the silica matrix membrane in the spin column in high-salt state, and then through a series of rapid rinsing-centrifugation steps, impurities such as salt, cell metabolites, and proteins are removed. Finally, a low-salt elution buffer elutes the λ phage DNA from the silica-based membrane. The obtained λ phage vector is widely used for library screening. AceSeq™ λ Phage DNA Kit rapidly isolates high-quality DNA free of hazardous chemicals and is ready to use. |
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Citation Guide |
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). |
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Application | For research use only |
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Product Type |
Nucleic Acid Extraction/Purification |
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Product Image |
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