Human Acute Myeloid Leukemia Peripheral Blood Mononuclear Cells (Relapsed/Refractory)

Description

Human Acute Myeloid Leukemia Peripheral Blood Mononuclear Cells (Relapsed/Refractory) are derived from the peripheral blood of patients with relapsed/refractory acute myeloid leukemia (AML). They are peripheral blood mononuclear cells primarily composed of leukemic myeloblasts.These cells achieve unlimited proliferation by abnormally activating signaling pathways such as RTK-RAS-MAPK and WNT/β-catenin, and inhibit the expression of myeloid differentiation genes through epigenetic remodeling (such as DNMT3A mutation) to maintain the original state . These cells frequently exhibit complex karyotypic abnormalities, including monosomy 7, trisomy 8, or translocations such as t(8;21).They often express CD33, CD34, and CD117, confirmed via flow cytometry.At the same time, they highly express CD47 and secrete immunosuppressive factors such as IL-6/TGF-β, which can inhibit macrophage phagocytosis and induce T cell exhaustion, and cooperate with stromal cells (MSCs) in the bone marrow microenvironment to build an immune escape niche.