Human MS Peripheral Blood Mononuclear Cells

Description

Human Multiple Sclerosis (MS) Peripheral Blood Mononuclear Cells (PBMCs) are isolated from the peripheral blood of MS patients using density gradient centrifugation and mainly include T cells, B cells, NK cells, monocytes, and dendritic cells. These cells play a central role in MS pathogenesis by contributing to inflammation, antigen presentation, and immune dysregulation. MS PBMCs show altered subsets, such as increased CCR7⁺IL-6⁺ T cells and decreased T-bet^hi CD4⁺ T cells, along with mitochondrial dysfunction and oxidative stress. Th17 cells and γδ T cells secrete IL-17A, driving neuroinflammation and CNS infiltration. Additionally, MS PBMCs secrete high levels of proinflammatory cytokines like IFN-γ and IL-6, while monocytes release regulatory cytokines such as IL-10. These features contribute to disease progression and offer potential targets for therapeutic intervention and biomarker development. Fresh MS PBMCs are available upon request for research applications. In advanced immunological investigations, these samples are widely used to analyze immune cell subsets and autoimmune markers, profile PBMC gene expression, monitor disease progression, and identify biomarkers associated with relapse and remission in multiple sclerosis.