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Immortalized Mouse Microglia | ||||
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| Product Name | Immortalized Mouse Microglia | |||
| Price | Get Quote | |||
| Cat.No | ABI-TC3325 | Species | Mouse | |
| Size/Quantity | 1 vial | |||
| Shipping Info | Dry Ice | Storage | Liquid Nitrogen | |
| Description |
Why choose Immortalized Mouse Microglia from AcceGen? Choosing Immortalized Mouse Microglia from AcceGen is a reliable decision due to their high quality and viability. AcceGen utilizes a standardized culture system and procedures to ensure consistent performance. Additionally, rigorous quality control measures are implemented, guaranteeing the reliability and reproducibility of the cells, making them an excellent choice for research and experimentation. | |||
| Disease | Normal | |||
| Source Organ | Brain | |||
| Quality Control | 1) Functional assays such as the nitrate assay was performed to assess nitric oxide production; 2) ELISA was used to determine the TNFα secretion after inflammatory stimulation; 3) Western blot and immunocytochemistry were used to confirm the expression of microglial specific markers such as CD68 and Iba1 and M1/M2 phenotype-associated protein expression (COX-2/iNOS and Arg-1) after stimulation; 4) Aβ1-42 or E. coli-derived bioparticles uptake was used to determine phagocytic activity of SIM-A9 cells. | |||
| Recommended Medium And Supplement | ABI-TM3325 Mouse Microglia Medium Kit | |||
| Citation Guide | When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID). | |||
| Application | FOR RESEARCH USE ONLY Immortalized mouse microglia, which closely resemble primary microglia, have significant applications as a model system for studying various aspects of microglial function. These cells can be used to investigate important characteristics such as stimulus-triggered microglial migration, proliferation, and phagocytosis. Furthermore, the activation of immortalized mouse microglia in response to exposure to HIV-Tat provides researchers with a valuable tool for studying HIV latency and regulation. By utilizing these immortalized cells, researchers can establish robust cellular models that offer insights into the mechanisms underlying HIV infection, latency, and regulatory processes, thus contributing to advancements in our understanding of neuroinflammatory diseases and potential therapeutic interventions. | |||
| Morphology | Heterogenous; spindle, multi-polar, spherical, flat | |||
| Immortalization Method | Spontaneous immortalization | |||
| Growth Conditions | 37 ℃, 5% CO2 | |||
| Cell Type | Microglia | |||
| Growth Mode | Adherent | |||
| Product Type | Immortalized Cell Line | |||
| Product Image |  | |||
Our immortalized mouse microglia are mouse microglia cells transformed using SV40 T antigen, allowing them to retain their properties indefinitely. These specialized macrophage cells are primarily located in the brain and spinal cord, serving as the first line of defense in the nervous system. Immortalized mouse microglia exhibit similar characteristics to primary microglia cells, expressing CD11b and Iba1, as confirmed by immunocytochemistry microphotographs and QPCR analysis. Moreover, they also demonstrate the expression of microglia-specific markers P2RY12 and TGFβR at the transcript level. When exposed to LPS treatment, these immortalized cells respond by releasing pro-inflammatory cytokines such as TNF-α, IL-1β, and IL-6.- ONLINE INQUIRY
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