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Human Pulmonary Artery Adventitia Fibroblasts

Product Name

Human Pulmonary Artery Adventitia Fibroblasts

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Cat.No

ABC-TC3773

Species

Human

Size/Quantity

1 vial

Biosafety Level

1

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

Fibroblasts are mesenchymal cells which derived from the embryonic mesoderm. They have been extensively used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different parts of the body are intrinsically different. The pulmonary arterial vesculature responds to various insults with a characteristic pattern of remodeling that includes an increase in connective tissue in the medial layer of the vessel wall. One of the pathogenetic mechanisms involved is the functional derangements of vascular fibroblasts. Their proliferative response contributes to the adventitial thickening observed during the development of hypoxia-induced pulmonary hypertension. HPAAF from AcceGen Research Laboratories are isolated from human pulmonary artery. HPAAF are cryopreserved at passage one and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HPAAF are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HPAAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAAF are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Research Laboratories.

Disease

Normal

Source Organ

Pulmonary Artery

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

Duncan Davis-Hall et al, 2023 Biofabrication, 15 015017DOI 10.1088/1758-5090/aca8cf.

Application

For research use only

Growth Conditions

37 ℃, 5% CO2

Cell Type

Fibroblast

Growth Mode

Adherent

Product Type

Pulmonary Cells

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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