MDCK Derivation and Characters Madin-Darby Canine Kidney cells (MDCK) are derived from kidney of normal female adult Cocker Spaniel canine. At first, MDCK heterogeneous was noticed due to the variable morphology, chromosome number from different laboratories analysis result. MDCK cells are then characterized by their growth, immunologic and cytogenetic properties, as well as their susceptibility to several viruses.
Why Choose MDCK from AcceGen? AcceGen MDCK cells are suitable for simulating epithelial tissue in vitro, as their high apico-basolateral polarity and tight junctions. MCDK and its sub-clones play important roles in epithelial cell trafficking, cell polarity and tight junctions. MDCK cell line is also a good choice for virus study and vaccine development.
Recommended Medium And Supplement
Eagle’s minimal essential medium with 10% fetal calf serum.
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For research use only MDCK is a commonly used epithelial cell model to study viral infection, environmental pollutants and vaccines production. MDCK cells are also widely used to isolate influenza viruses due to their high susceptibility to infection with various influenza strains.
In the last few decades, MDCK cells were found to proliferate, form hollow tubules in monolayers and respond to the added collagen. Such discoveries suggest the possibility of a MDCK 3D culture.
Moreover, several strains with different resistive and transport properties have been derived from MDCK for different research purposes.
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Canine Kidney Cell Lines
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