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Tumor Cell Lines

NIH-3T3

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    1

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Mouse Swiss NIH embryo. Established from a NIH Swiss mouse embryo. These cells are sensitive to sarcoma virus focus formation and leukemia virus propogation. Cells have now lost their contact inhibition.
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Product Code

NIH/3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3

Species

Mouse

Cat.No

ABC-TC0828

Product Category Tumor Cell Lines
Size/Quantity

1 vial

Cell Type

Fibroblast

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Embryo

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Mouse Embryo Cell Lines

Description

NIH-3T3NIH-3T3 is a mouse embryonic fibroblast cell line that was originally isolated from NIH/Swiss mouse embryos. NIH-3T3 exhibits fibroblast-like morphology and adherent culture properties. NIH-3T3 is a hypertriploid mouse cell line, and it has tested negative for the ectromelia virus (mousepox). NIH-3T3 displays identifiable clonal abnormalities and is highly susceptible to cellular transformation.

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Citation

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Application

  • The NIH-3T3 cell line is a widely used fibroblast model in molecular and cellular biology. Known for its spontaneous immortalization, this mouse embryonic cell line is commonly used in DNA transfection experiments and tumor DNA transfer studies. NIH-3T3 cells enable the identification of transforming oncogenes and support investigations into the molecular mechanisms of cellular transformation and cancer development.

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