Tumor Cell Lines

NIH-3T3

  • For research use only

Cat No.

ABC-TC0828

Product Type

Mouse Embryo Cell Lines

Cell Type

Fibroblast

Species

Mouse

Growth Conditions

37 ℃, 5% CO2

Source Organ

Embryo

Disease

Normal

Product Code

NIH/3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3

NIH-3T3 is a mouse fibroblast cell line, which is widely used in oncogene expression, transformation assays, and tissue remodeling research.

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Description

NIH-3T3 (also commonly abbreviated as NIH3T3) is a mouse embryonic fibroblast cell line established from NIH Swiss mouse embryos. These adherent NIH 3T3 fibroblasts display a spindle-shaped, fibroblast-like morphology and are known for their contact inhibition and stable diploid karyotype. NIH-3T3 cells express fibroblast markers such as vimentin and fibronectin, and demonstrate consistent proliferation under standard culture conditions. Their gene expression profile includes regulators of the cell cycle, extracellular matrix, and key signaling pathways related to growth and differentiation. Due to their genetic stability, high transfection efficiency, and ease of maintenance, NIH-3T3 cells are a classic model for studying fibroblast biology, oncogenic transformation, and gene function. They are widely used in focus formation assays and serve as a standard host in evaluating tumorigenic potential of oncogenes and signaling molecules.

Product Code

NIH/3T3; NIH3T3; 3T3; 3T3NIH; 3T3-Swiss; Swiss-3T3; Swiss/3T3; Swiss 3T3; Swiss3T3

Species

Mouse

Cat.No

ABC-TC0828

Product Category

Tumor Cell Lines

Size/Quantity

1 vial

Cell Type

Fibroblast

Growth Mode

Adherent

Shipping Info

Dry Ice

Growth Conditions

37 ℃, 5% CO2

Source Organ

Embryo

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Mouse Embryo Cell Lines

Application

  • The NIH-3T3 cell line is a widely used fibroblast model in molecular and cellular biology. Known for its spontaneous immortalization, this mouse embryonic cell line is commonly used in DNA transfection experiments and tumor DNA transfer studies. NIH-3T3 cells enable the identification of transforming oncogenes and support investigations into the molecular mechanisms of cellular transformation and cancer development.

Citation

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

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High Viability
To succeed in cell culture
Precision and Reliability
To support a consistent result
Customization Options
Tailed to your research

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