Immortalized Human Liver Stellate Cells

Frequently Asked Questions

• What are the key characteristics of Immortalized Human Liver Stellate Cells?

  Immortalized Human Liver Stellate Cells are derived from human liver and have been immortalized using Lenti-SV40T Lentivirus. They can go beyond 30 passages, unlike primary cells that senesce after the 3rd passage. These cells express markers like vimentin and α-actin and are important for studying liver extracellular matrix homeostasis, repair, regeneration, fibrosis, and retinol metabolism.

• How should the Immortalized Human Liver Stellate Cells be cultured and maintained?

  These cells should be cultured in Human Liver Stellate Cells Medium and grown in poly-L-lysine coated flasks. For initial culturing, the medium should be changed the day after thawing to remove residual DMSO and unattached cells.

• What are the recommended storage and handling guidelines for these cells?

  The cells are stored in liquid nitrogen and should be handled delicately. Upon receiving, they should be thawed quickly at 37ºC and transferred to culture with minimal handling to avoid damage. It is recommended not to centrifuge or dilute the cells right after thawing to minimize harm.

• What are the applications of these Immortalized Human Liver Stellate Cells in research?

  These cells are used to study various aspects of liver disease, including inflammation, fibrogenesis, and the pathogenesis of intrahepatic portal hypertension. They are pivotal for understanding the dynamics of liver fibrosis and the role of hepatic stellate cells in liver injury and repair.

• What are the safety considerations when handling these cells?

  It is crucial to follow universal precautions for handling human-derived products, including wearing gloves and safety glasses, and never using mouth pipetting techniques to avoid exposure to potential biohazards.