For research use only
| Cat No. | ABC-TC0862 |
| Product Type | Human Gastric Cancer Cell Lines |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Lymph Node |
| Product Code | NUGC4; NU-GC-4 |
NUGC-4 gastric cancer cells from Japanese female show epithelial morphology,anchorage-independent growth,unique IL-1α expression, ideal for tumor studies.
NUGC-4 cell line is a human gastric cancer model derived from metastatic lymph node tissue of a 35-year-old Japanese female with poorly differentiated adenocarcinoma containing signet ring cell components. These epithelial-like cells exhibit spherical morphology with predominant adherent growth (occasional free-floating cells) and demonstrate anchorage-independent proliferation, confirming their tumorigenic potential. They maintain a stable karyotype (modal chromosome number 52-54 in vitro, 53 in vivo) and rapid doubling time under standard culture condition. Additionally, NUGC-4 lacks IL-1α mRNA expression – a characteristic feature of its diffuse-type gastric cancer origin – making it particularly valuable for studying IL-1-independent inflammatory pathways in tumor microenvironments. This cell line’s aggressive phenotype, combined with its unique molecular profile (including E-cadherin methylation), establishes it as an ideal system for investigating gastric cancer metastasis, drug resistance mechanisms, and tumor-stroma interactions in both in vitro and in vivo models.
| Product Code | NUGC4; NU-GC-4 |
| Species | Human |
| Cat.No | ABC-TC0862 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Lymph Node |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Gastric Cancer Cell Lines |
NUGC-4 is widely used to identify key factors in gastric cancer formation and targets for anti-cancer drugs. Epidermal growth factor (EGF) strongly activates the tyrosine phosphorylation in NUGC-4. Phosphatidylinositol 3-kinase (PI 3-kinase), mainly activated by tyrosine kinases, was precipitated with anti-phosphotyrosine antibodies. Exogenous hepatocyte growth factor can stimulate the proliferation of NUGC-4, and MET tyrosine kinase inhibitors inhibited this proliferation of NUGC-4. Investigation of NUGC-4 and its interactions with growth factors may guide gastric cancer signaling pathways and therapeutic target identification.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
NUGC-4 cells are a human gastric cancer cell line derived from a poorly differentiated adenocarcinoma. They are widely used in cancer research, particularly for studies focused on gastric carcinoma, drug screening, and understanding cancer cell biology.
Slow Growth: Ensure regular medium change, check for contamination, and avoid over-confluency.
Poor Attachment: Use properly coated culture flasks and high-quality serum.
Cell Death: Maintain optimal culture conditions, verify reagents are non-toxic, and handle cells gently.
Cell Shape: NUGC-4 cells are epithelial-like in morphology. They typically appear as polygonal or slightly elongated cells when observed under a microscope.
Adherence: They adhere well to the surface of culture flasks, forming a cobblestone-like pattern characteristic of epithelial cells.
Cytoplasm and Nucleus: The cells have a well-defined cytoplasm and prominent nuclei, which are indicative of their active proliferative state.
