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SF295; SF.295; SF 295






1 vial

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Liquid Nitrogen


SF-295 cells are human glioblastoma cells derived from central nervous system carcinoma. SF-295 is a patient-derived glioblastoma cell line originating from a temporal lobe tumor. SF295 cells do not express the glial markers GFAP or glutamine synthetase, but have been shown to positively express neurofibromin-1, identified as one of the most frequently mutated factors in glioblastoma multiforme. SF295 cells exhibit hypertriploidy and glial-like morphology in culture and are non-tumorigenic in athymic mice. SF-295 is identified to be homozygous for PTEN and TP53 and have a late-stage phenotype.


Why choose SF-295 from AcceGen?

Choosing SF-295 from AcceGen offers several advantages. Firstly, each vial of SF-295 contains a substantial quantity of over 1×106 viable cells, ensuring an ample supply for experiments. Secondly, these cells are rigorously tested for infectious diseases, ensuring their safety and reliability. Additionally, SF-295 cells are confirmed to be free from mycoplasma contamination, further enhancing their suitability for experimentation. The unique identity of the cell line is verified through genotyping using STR analysis for each lot. Lastly, SF-295 cells from AcceGen have a minimum of 15 population doubling levels when cultured following our protocol, indicating their robust growth potential for long-term studies.

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Citation Guide

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The SF-295 cell line finds extensive applications in brain cancer research, particularly focused on glioblastoma multiforme (GBM), the most aggressive and malignant form of brain cancer. It serves as a standard brain tumor model in various cell line panel projects, making it invaluable in contemporary research. SF-295 is highly utilized in studies investigating chemotherapeutic treatments for glioblastoma, providing a valuable cellular model for assessing the impact of drugs and molecular modulators on glioblastomas. Through these applications, SF-295 contributes significantly to advancing our understanding and developing potential therapeutic interventions for this devastating disease.

Growth Conditions

37 ℃, 5% CO2

Cell Type


Growth Mode


Product Type

Human Nerve Tumors Cell Lines

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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