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Host Cell Information Survey

Project type

Knockout Knockin Knockdown Overexpression Point Mutation

Gene name

Gene ID: Gene name:

Cell name

Cell origin

Laboratory inventory Third-party

Do the cells contain any pathogens that could be harmful to humans?

Yes No

Cell count

Quantity (vial)

Subculture frequency

Cell type

Infinite cell line Finite cell line

Cell-passage

Cell passaging ability:

<10 10-20 30 Infinite proliferation Unknown

Passage number now:

Medium

DMEM low glucose DMEM high glucose RPMI-1640 DMEM/F12 Other:

Serum concentration:

Antibiotic type and concentration

Specific culture conditions

CO₂ concentration

Temperature

℃

Growth type

Adherent Suspension Semi-adherent Others

Microbe test

Bacteria Mycoplasma Human pathogen Both not

Transfection test

Not tested Tested Transfect method: Results:%

Monoclone forming Capability

Strong Weak Untested

Gene editing

Not edited (Wild type) Edited (Resistance gene, fluorescence gene…)
Description:

Reagents for cryopreservation

Which type of cells do you want?

a cell pool a single clone other:

Which gene expression verification method do you want? (Extra fees may be charged)

qPCR Sequencing Flow cytometry (Please provide antibody) Western blotting (Please provide antibody)
Off-target analysis other:

Could you please describe the application to the cell line?

Cryopreserved cells: 1.5-1.8 ml in cryopreservation vial, at least 1 × 106 cells per vial. It is recommended to culture cells until logarithmic growth stage in 25cm2 flask, and each flask is frozen into 1 vial.
Enough dry ice is filled in the foam box for delivery. Cells frozen within 2 months is recommended.
The provided host cells should be in good condition, free of contamination and suitable for transfection.
Please be sure of the accuracy and authenticity of the information provided.

Cell pictures and other information

1.Please provide the following pictures (optional)

Cell culture:

Monoclonal cell:

Transfection:

2.Please provide literature if any. (picture, word, pdf)

* Please submit literature directly by sending an email to inquiry@accegen.com if the file is over 5M in total.

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