Hepatocellular carcinoma (HCC) is one of the lethal cancers with a dramatically rising incidence rate. HCC cell lines are widely used in various types of liver cancer research. Genomes of hepatocellular carcinoma cell lines are different from those of healthy human cells. Common homo sapiens hepatocellular cancer cell lines include PLC, Huh7, Huh7.5, Hep3B, HepG2, SMMC-7721, MHCC97-H, MHCC9-L and so on; and each cell line has its own characteristics. Therefore, the genome and hepatotoxicity of hepatocellular carcinoma cell lines are very significative in researching the occurrence and development of hepatocellular carcinoma.
The hepatocellular carcinoma cell line is a cell group formed by the continuous division and proliferation of a single cell after isolation from the pathological tissues of hepatocellular carcinoma. It has the characteristics very similar to the original tissues and the complete genetic material of the human body and can be passed on steadily. Human hepatocellular carcinoma cell lines have different names because of the parental patients from which they were isolated. The common hepatocellular carcinoma cell lines are PLC, Huh7, Huh7.5, Hep3B, HepG2, SMMC-7721, MHCC97-H, MHCC9-L and so on; and these hepatocellular carcinoma cell lines have been studied in the aspects of etiology, pathogenesis, diagnosis, treatment, drug screening, animal experiments and so on. Hepatocellular carcinoma (HCC) cell lines are widely used. Because they have similar characteristics to original HCC cells, i.e. no biological changes, stable characteristics, and unlimited passage, they provide an ideal situation in vitro model for the study of HCC and become another favorite replacement in the study of HCC diseases except conducting animal experiments. The characteristics of several human hepatocellular carcinoma cell lines and their latest research were collated to provide a reference for oncologists’ experimental research. So, they are suitable for hepatocellular carcinoma research.
application:Huh-7 cell lines commonly used to conduct research on the pathogenesis of hepatocellular carcinoma and it may represent a convenient and affordable alternative to the use of human hepatocytes.
Figure 1. The Cultivating morphology of representative Huh7 cells can last for 7 days (A) or 28 days (B), where the Huh7 cells appeared as a homogeneous cell population with an epithelial phenotype showing no regular structural organization. After the cells reached confluency, they underwent morphological changes and took on the appearance of cuboidal cells, hepatocyte-like cells, and spherical actin filament bundles. After 4 weeks of culturing, the cells had organized into two different clusters.
application: HepG2 human hepatocellular carcinoma cell line has been widely used in the physiological study of the liver, but the expression levels of liver-specific drug-metabolizing enzymes and transcription factors are low.
application: The human hepatoma Hep3B cell line has long been used to test the efficacy of therapeutic agents, vitro cytotoxicity, DNA synthesis, and caspase activity.
Figure 2. Hep3B cellular morphology observed after treatment of the cells with BI 2536, a specific Plk1 inhi -bitor
Human hepatocarcinoma cells are mostly derived cells from primary liver cancer tissues. By culturing hepatocellular carcinoma cells and analyzing them at a single-cell level, it is possible to understand the subtleties of the formation of hepatocellular carcinoma in more detail and apply them to the study of hepatocellular carcinoma biology. One advantage of using in vitro systems for research is that they can be done faster and cheaper than in vivo studies. For example, if you want to do experiments on the proliferation of hepatocellular carcinoma cells, you can choose HepG2 or Hep3B hepatocellular carcinoma cells instead of spending too much time choosing them. The genome and cytotoxicity of HepG2, Huh7, and Hep3B have been researched deeply, as they are essential for the development of hepatocellular carcinoma diseases and drug development in the later stage.
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