For research use only
| Cat No. | ABC-TC0381 |
| Product Type | Human Liver Cell Lines |
| Cell Type | Epithelial |
| Species | Human |
| Growth Conditions | 37 ℃, 5% CO2 |
| Product Code | HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 |
Leverage Hep-G2 cells to study hepatic metabolism, oxidative stress responses, and lipoprotein gene regulation in liver function and disease research.
Hep G2 cells are a human liver-derived epithelial cell line originally established from a liver tumor of a 15-year-old male patient. The cells exhibit adherent, epithelial-like morphology and retain several hepatocyte-associated functions and markers, including albumin and alpha-fetoprotein (AFP) expression. Hep G2 cells have been widely used as an in vitro model for studying liver biology, cancer-related mechanisms, xenobiotic responses, and cellular toxicity. However, compared with primary human hepatocytes, Hep G2 cells have relatively limited expression and activity of several drug-metabolizing enzymes, which should be considered when interpreting metabolism and toxicity data.
| Product Code | HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 |
| Species | Human |
| Cat.No | ABC-TC0381 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Liver Cell Lines |
Hep G2 cells can be used for liver tumor biology, hepatoblastoma-related research, hepatotoxicity assessment, drug response studies, oxidative stress research, and investigations of hepatic cellular functions. The cells are widely used in in vitro studies evaluating compound-induced cytotoxicity and cellular responses to environmental or chemical stressors. Hep G2 cells can also support research on lipid metabolism, hepatic gene regulation, and drug-related cellular responses. Their compatibility with high-throughput and multiwell assay formats makes them useful for screening studies, although results involving drug metabolism should be interpreted with consideration of their relatively low cytochrome P450 expression compared with primary human hepatocytes.
Research using Hep G2 cells focuses on liver tumor biology and hepatoblastoma-related biology, hepatic metabolism, drug-induced liver injury, oxidative stress, lipid metabolism, and cellular responses to xenobiotics. Hep G2 cells have been widely incorporated into hepatotoxicity research because they are readily available, proliferative, and amenable to standardized in vitro assays. The model can also be used in studies of oxidative stress and antioxidant responses, as well as molecular mechanisms associated with liver disease and cancer. For studies requiring physiologically representative hepatic drug metabolism, Hep G2 results should be complemented with more metabolically competent models such as primary human hepatocytes or appropriately engineered hepatic cell systems.
Cell Recovery(Thaw and recover the cryopreserved Hep G2 cells under appropriate culture conditions.) → Cell Culture(Maintain the adherent epithelial cells under the recommended culture conditions.) → Cell Expansion(Expand the cells to obtain sufficient material for downstream experiments.) → Experimental Treatment(Expose Hep G2 cells to candidate compounds, drugs, environmental chemicals, or other experimental conditions.) → Cellular Response Assessment(Evaluate cell viability, proliferation, morphology, oxidative stress, or other treatment-associated responses.) → Molecular Analysis(Analyze gene expression, protein expression, signaling pathways, or metabolic markers associated with hepatic responses.) → Drug Response Analysis(Assess concentration-dependent or treatment-associated cellular responses to investigate drug effects or potential hepatotoxicity.) → Data Interpretation(Integrate phenotypic and molecular results to characterize hepatic cellular responses and evaluate experimental hypotheses.)
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $200 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Ding L, Liang X. Ras related GTP binding D promotes aerobic glycolysis of hepatocellular carcinoma. Ann Hepatol. 2021;23:100307. doi:10.1016/j.aohep.2021.100307
Al-Mahbashi HM, Al-Shamahy HA. Cytotoxic Activities in Vitro of Flower Extracts of Three Species of Aloe Growing in Yemen: Aloe Rubroviolaceae, Aloe Vera and Aloe Sabaea, against Eleven Types of Cancer Cell Lines. Journal of Clinical Research and Reports. 2021;08(05):01-10. doi:10.31579/2690-1919/187
