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| Product Code | HEP-G2; Hep G2; HEP G2; HepG2; HEPG2 |
| Species | Human |
| Cat.No | ABC-TC0381 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Epithelial |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Human Liver Cell Lines |
Hep-G2 is a human hepatoblastoma cell line derived from the liver tumor of a 15-year-old Caucasian male patient. These epithelial-like cells exhibit adherent growth properties and have a doubling time ranging from 48-72 hours under standard DMEM culture conditions. The karyotype is hyperdiploid, with a modal chromosome number of 55 and structural abnormalities including chromosome 1/21 translocations. Hep-G2 retains hepatocyte-specific functions, such as the expression of albumin, alpha-fetoprotein, and key hepatic metabolic enzymes like 3-hydroxy-3-methylglutaryl-CoA reductase. The cell line shows a TERT promoter mutation (C228T) commonly found in hepatocellular carcinomas. in contrast to its HBV-expressing subclone Hep-G2/2.2.15, the parental Hep-G2 line demonstrates low tumorigenicity in immunocompromised mice.
When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Ding, L. and Liang, X., 2021. Ras related GTP binding D promotes aerobic glycolysis of hepatocellular carcinoma. Annals of Hepatology, 23, p.100307.
The Hep-G2 cell line, is widely utilized in pharmacological, toxicological, and liver function research, particularly for studies related to xenobiotic metabolism and drug elimination. Despite certain enzymatic limitations compared to primary hepatocytes, Hep-G2 cells remains a promise model in drug metabolism investigations and hepatotoxicity assessments, owing to its expression of drug metabolizing-enzymes and transport proteins relevant to both hepatocellular carcinoma and hepatoblastoma. Recent research efforts focused on amplifying the cytochrome P450 expression within Hep-G2 cells to enhance their utility as a hepatocyte model. Furthermore, 3D spheroid culture systems are being actively explored to establish a more physiologically relevant model, as these models demonstrate greater metabolic activity compared to conventional 2D cell cultures.
