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BT 549; BT.549; BT549






1 vial

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Dry Ice


Liquid Nitrogen


The BT-549 cell line, also known as HTB-122, originates from an invasive ductal tumor in the breast of a 72-year-old female cancer patient with extensive lymph node metastasis. Isolated in 1978, these epithelial cells maintain typical breast and mammary gland tissue characteristics. Notably, BT-549 cells exhibit aneuploidy with a modal chromosome count of 78, ranging from 73 to 80, and aberrations in the X chromosomes. Chromosomes N10 and N13 are underrepresented, while N2, N12, N17, and N8 deviate from normal copy numbers. Chromosomes N5 and N18 typically display increased copies. Additionally, BT-549 cells carry four marker chromosomes: iso(13q), 10q+, del(x)(q22:), and del(11)(p11:). These cells have a doubling time of around 3.7 days.


Why choose BT-549 from AcceGen?

The BT-549 cell line from AcceGen offers high viability and quality, having been cryopreserved using advanced techniques. These cells are maintained under optimal conditions, ensuring their sterility as they are rigorously screened for the absence of bacteria, fungi, and mycoplasma, meeting stringent quality control standards.


Ductal Cancer

Source Organ


Recommended Medium And Supplement
Citation Guide

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The BT-549 cell line has emerged as a valuable asset in biological science, primarily within the realms of cancer research and 3D cell culture applications. Its significance lies in its relevance to ductal carcinoma, particularly in gaining insights into its mechanisms and potential therapeutic targets. As a pivotal component of cancer research, BT-549 cells enable a deeper understanding of carcinoma development and the exploration of innovative treatment strategies. Moreover, their applicability in 3D cell culture makes them an ideal tool for simulating the intricate tumor microenvironment, facilitating the study of drug responses and tumor progression in a more physiologically relevant context.

Growth Conditions

37 ℃, 5% CO2

Cell Type


Growth Mode


Product Type

Human Breast Cancer Cell Lines

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