The C57BL/6 mouse esophageal epithelial cell line is derived from the esophagus of healthy wild-type mice, without disease background. They retain typical epithelial markers, including E-cadherin and ZO-1, confirmed via immunofluorescence. The cells show a normal diploid karyotype (2n=40), verified by G-banding, and are recommended for use below passage 20 to maintain phenotype. Non-tumorigenic and non-metastatic, they are free of mouse-specific viruses such as ectromelia and mouse hepatitis virus. This cell line is widely used to study esophageal epithelial-stromal interactions and diseases such as eosinophilic esophagitis. Its non-cancerous, genetically stable nature makes it ideal for investigating fibrotic responses and epithelial barrier dysfunction in esophageal tissues.
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