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CHO K1; CHOK1; CHO cell clone K1




Hamster, Chinese


1 vial

Biosafety Level


Shipping Info

Dry Ice


Liquid Nitrogen


CHO-K1 cells are a subclone of the CHO cell line, derived from an adult Chinese hamster ovary biopsy in 1957. These diploid cells share a karyotype similar to human cells and have a genomic sequence of 2.45 Gb with 24,383 predicted genes. Notably, CHO-K1 cells lack the gene for proline synthesis, requiring proline for growth. They exhibit rapid growth, ease of culture, and minimal chromosomal abnormalities. Interestingly, these cells show morphological changes in response to cholera toxin. While CHO-K1 cells are susceptible to certain viruses, they display resistance to Poliovirus, Modoc virus, and Buttonwillow virus. The presence of numerous glycosylation genes affects the quality of therapeutic proteins and their susceptibility to viruses.


Why choose CHO-K1 from AcceGen?

CHO-K1 cells from AcceGen are an excellent choice due to their production and cryopreservation carried out by professional operators using advanced techniques under standardized conditions. These cells boast high quality, viability, and undergo rigorous control measures to ensure their reliability and consistency in various applications.

Source Organ


Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).
Ng, J.Y., Chua, M.L., Zhang, C., Hong, S., Kumar, Y., Gokhale, R. and Ee, P.L.R., 2020. Chlorella vulgaris extract as a serum replacement that enhances mammalian cell growth and protein expression. Frontiers in bioengineering and biotechnology, 8, p.1068.



The CHO-K1 cell line can be genetically modified to grow as adherent or suspension cells and have gained regulatory approval for the production of therapeutic recombinant proteins. CHO-K1 cells serve as an excellent host expression system for various products, including growth factors, hormones, monoclonal antibodies, interferons, and enzymes. Moreover, they are utilized in in vitro cancer research, especially for studying ovarian cancer. The popularity of CHO-K1 cells in protein production stems from their favorable attributes such as high growth rate, ease of culture, and low occurrence of chromosomal abnormalities.

Growth Conditions

37 ℃, 5% CO2

Cell Type


Growth Mode


Product Type

Mouse Ovarian Cell Lines

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cho-k1 cell line

AcceGen product cho-k1 cell line

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