Human Pericardial Fibroblasts

Description

Human Pericardial Fibroblasts are primary stromal cells isolated from the parietal or visceral layers of the pericardium of healthy adult donors. These mesenchymal cells exhibit a characteristic spindle-shaped morphology and adhere to culture dishes. They demonstrate robust extracellular matrix (ECM) production, including collagen types I and III, fibronectin, and periostin—key components involved in cardiac fibroblast function during tissue remodeling and wound repair responses. They constitutively express vimentin and discoidin domain receptor 2 (DDR2), and upon TGF-β1 stimulation, undergo myofibroblast transition as evidenced by α-smooth muscle actin (α-SMA) upregulation. These cells are cryopreserved at an early passage to retain their physiological phenotype. As non-transformed primary cells, they have finite proliferative capacity and are not suitable for long-term passaging. Each lot undergoes rigorous screening and isolation procedures, and is rigorously tested to ensure it is free of contamination from HIV-1, HBV, HCV, Syphilis, Mycoplasma, Fungi, Yeast, and Bacteria.