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Immortalized Mouse Osteocytic Cell Line-Conditionally

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Immortalized Mouse Osteocytic Cell Line-Conditionally

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1 vial

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Immortalized Mouse Osteocytic Cell Line represent a non-homogenous population progressing from early osteoblasts to late osteocytic. These cells express functional SV40 large T antigen that is induced in the presence of IFN γ under permissive temperature (33°C) and express GFP under control of the dentin matrix (Dmp1) promoter. Initial culture shows osteoblastic phenotype, however when under mineralizing conditions, the cells start to express early osteocyte markers such as E11/podoplanin, followed by Dmp1 and mature markers SOST and FGF23 by 21-28 days of culture.
Similar to osteocytes in vivo, these cells respond to hormonal signals such as PTH and 1,25-dihydroxyvitamin-D3. Immortalized Mouse Osteocytic Cell Line can be maintained in both 2D and 3D cultures and are useful for study of osteocytes at various differentiation stages. It should be noted that these cultures may contain a mixture of osteoblasts and osteocytes at different stages of differentiation and for studies requiring enriched osteocytes, the Dmp1-GFP cells should be isolated by flow cytometry (FACS).



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1) Presence and absence of SV40 large T-antigen, under permissive and non-permissive conditions, were confirmed using western blot. 2) Fluorescent imaging was used to measure Dmp1 promoter driven GFP expression in osteogenic conditions. 3) Von Kossa staining was used to show focal nodular mineralization and Alizarin red S staining was used to detect calcium deposition of the differentiated cells.

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For research use only



Immortalization Method

Isolated from long bones of transgenic mice (in which the Dmp1 promoter drives the expression of GFP).

Growth Conditions

37 ℃, 5% CO2

Cell Type

Osteocytic Cell

Growth Mode


Product Type

Immortalized Cell Line

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