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Immortalized Human Lens Epithelial Cell Line – SV40

Product Name

Immortalized Human Lens Epithelial Cell Line – SV40

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Cat.No

ABI-TC170D

Species

Human

Size/Quantity

1 vial

Biosafety Level

2

Shipping Info

Dry Ice

Storage

Liquid Nitrogen

Description

The Immortalised Lens Epithelial Cells – SV40 is established from primary cells (isolated from infant lens epithelial fragments) using Adl2-SV40 adenovirus. The Immortalised Human Lens Epithelial Cells – SV40 provides a homogeneous population of cell for the investigation of the physiology of the normal human lens epithelium and study its response to cataractogenic agents and the human lens specific functions. These cells also provide a unique system for examining the relationship between gene expression and differentiation.

Disease

Normal

Source Organ

Eye

Quality Control

The Immortalized Human Lens Epithelial Cells react positively with the monoclonal antibody to SV40 large T antigen. Western blot and precipitation are used to confirm the presence of βH- and γ-crystallin, the proteins that are markers for cell differentiation in the lens epithelium. SV40 was observed through IHC (Figure 6).

Recommended Medium And Supplement
Citation Guide

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Application

For research use only

Morphology

Polygonal

Immortalization Method

Serial passaging and transduction with recombinant adenovirus carrying SV40 Large T antigen

Growth Conditions

37 ℃, 5% CO2

Cell Type

Epithelial

Growth Mode

Adherent

Product Type

Immortalized Cell Line

Product Image AcceGen Frozen Cells & Cell Lines 1 vial
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