Home > Products > Tumor Cell Lines > Human Tumor Cell Lines >


Product Name


Price Get Quote
Product Code

A 549; A-549; NCI-A549; hA549






1 vial

Biosafety Level


Shipping Info

Dry Ice


Liquid Nitrogen


The A549 cell line, established in 1972, originates from the lung adenocarcinoma of a Caucasian male. These hypotriploid alveolar basal epithelial cells have a modal chromosome number of 66, with occasional variations. A549 cells express keratin and have the capacity to synthesize lecithin and contain unsaturated fatty acids. They are squamous in nature and play a role in facilitating the diffusion of substances across the lung’s alveoli. In laboratory settings, A549 cells grow as adherent monolayers. They thrive in a nutrient-rich DMEM culture medium supplemented with fetal bovine serum or F12-K medium with 10% non-heat inactivated fetal bovine serum.


Why choose A549 from AcceGen?

AcceGen’s A549 cells offer several compelling reasons to choose them for your research needs. Firstly, they ensure high quality and viability, providing reliable and consistent results. Secondly, these cells are guaranteed to be sterile and free from mycoplasma contamination, minimizing the risk of experimental interference. Additionally, AcceGen provides optimal incubation conditions, allowing for the cells’ optimal growth and functionality.


Lung Cancer

Source Organ


Recommended Medium And Supplement
Citation Guide

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).



The A549 cell line, which has been approved by the FDA, finds applications in various fields. One major application is in adenovirus production, including the replication of adenovirus constructs that do not require complementation by the viral oncogene EA1. A549 cells are also used for disease modeling, particularly for studying the metabolic processing of lung tissue and mechanisms of drug delivery. They have been employed in researching lung cancer, allergies, asthma, and respiratory infections. In drug development, A549 cells are utilized for in vitro and in vivo testing of novel drugs, including xenograft lung cancer models. Furthermore, A549 cells have been used in enzymatic studies to investigate cancer cell proliferation and the effects of insulin. Additionally, they serve as a host for CRISPR Cas9 delivery, enabling precise DNA editing.

Growth Conditions

37 ℃, 5% CO2

Cell Type


Growth Mode


Product Type

Human Lung Cancer Cell Lines

Product Image

a549 cell lineAcceGen product a549 cell line

Related Resources

When you publish your research, please cite our product as “AcceGen Biotech Cat.# XXX-0000”. In return, we’ll give you a $100 coupon. Simply click here and submit your paper’s PubMed ID (PMID).

We know how valuable your research is to you, but are you wondering what you can expect to pay for quick accurate results every time?

Fill out a request in the form below and we’ll get back to you within 24 hours with a quote.
Privacy Policy: AcceGen will never sell, rent, or share your personal information with any third parties without your express permission.
Reviews of A549
AcceGen is always trying to do right by our customers and working hard to build a higher quality product.

Your email address will not be published.

AcceGen Scroll Top Button