AR Knockout 293T Cell Line

Description

AR Knockout 293T Cell Line is generated from human embryonic kidney 293T cells through CRISPR/Cas9-mediated disruption of the Androgen Receptor (AR/NR3C4) gene, a ligand-activated transcription factor critical for male sexual development and prostate cancer progression. This knockout model exhibits complete loss of androgen-responsive gene regulation while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line serves as an essential tool for studying androgen signaling pathways, prostate cancer mechanisms, and therapeutic resistance, particularly in research involving enzalutamide sensitivity and AR splice variants. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through multiplex analysis including genomic PCR, Sanger sequencing, and luciferase reporter assays demonstrating abolished response to dihydrotestosterone (DHT). Rigorous quality control confirms the cell line is free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.