Axin1 Knockout 293T Cell Line

Description

Axin1 Knockout 293T Cell Line is engineered from human embryonic kidney 293T cells using CRISPR/Cas9 technology to disrupt the Axis Inhibition Protein 1 (Axin1) gene, a critical scaffold protein in the Wnt/β-catenin signaling pathway that regulates cell proliferation, differentiation, and embryonic development. This knockout model exhibits hyperactivated Wnt signaling with consequent β-catenin stabilization while maintaining 293T cells’ characteristic adherent growth with epithelial morphology and high transfection efficiency. The cell line provides a powerful tool for studying Wnt pathway dysregulation, carcinogenesis, and stem cell maintenance, particularly in research involving tumor formation and tissue regeneration. Maintained at low passage numbers (<P20) with genetic stability, the knockout efficiency is validated through genomic PCR, Sanger sequencing, and Western blot analysis of β-catenin accumulation. The cell line has undergone rigorous quality control and is certified free of contamination from HIV-1, HBV, HCV, Syphilis, mycoplasma, fungi, yeast, and bacteria.