B16-OVA Luciferase Reporter Cell Line

Frequently Asked Questions

• What is the B16-OVA Luciferase Reporter Cell Line?

  The B16-OVA Luciferase Reporter Cell Line is generated from the B16-OVA melanoma cell line, which is a mouse skin melanoma cell line that has been engineered to express both the ovalbumin (OVA) antigen and the luciferase gene. This cell line allows for the monitoring of tumor growth and immune response in vivo using bioluminescent imaging, making it particularly useful for cancer immunotherapy research.

• What is the recommended culture medium for this cell line?

  The B16-OVA Luciferase Reporter Cell Line should be cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. The cell line is also puromycin-resistant, and puromycin can be added to the medium at a concentration of 1µg/mL as needed for selection purposes.

• What are the specific applications of the B16-OVA Luciferase Reporter Cell Line?

  This cell line is extensively used for research in tumor immunology, particularly for studying the efficacy of immunotherapeutic agents in melanoma. Its expression of luciferase facilitates the real-time tracking of tumor progression and regression in animal models.

• What should you do upon the recepit of the B16-OVA Luciferase Reporter Cell Line?

  Upon receiving the cryovial, it should be thawed rapidly in a 37°C water bath, ensuring the cap remains above the water to avoid contamination. After thawing, cells should be transferred to a centrifuge tube containing complete culture medium, centrifuged, and the supernatant discarded. Cells should then be resuspended in fresh medium, transferred to a new culture flask, and placed in a 37°C CO2 incubator for further cultivation.

• What are the safety considerations when handling this cell line?

  Handling of the B16-OVA Luciferase Reporter Cell Line should adhere to Biosafety Level 1 (BLS-1). Personal protective equipment should be worn, and all procedures should be conducted in a biosafety cabinet to prevent contamination and ensure the safety of the handler. The cells are intended for research use only and should not be used for human or veterinary applications. Proper biocontainment and waste disposal procedures should be followed to mitigate any environmental impact.