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| Product Code | C2c12; C2-C12; C12 |
| Species | Mouse |
| Cat.No | ABC-TC0091 |
| Product Category | Tumor Cell Lines |
| Size/Quantity | 1 vial |
| Cell Type | Myoblast |
| Shipping Info | Dry Ice |
| Growth Conditions | 37 ℃, 5% CO2 |
| Source Organ | Muscle |
| Disease | Normal |
| Biosafety Level | 1 |
| Storage | Liquid Nitrogen |
| Product Type | Mouse Muscle Cell Lines |
C2C12 is a spontaneously immortalized murine myoblast cell line derived from the skeletal muscle of a 2-month-old female C3H mouse. It exhibits fusiform or star-shaped morphology in its undifferentiated state, transitioning into long, multinucleated myotubes during differentiation, expressing myofilament proteins such as actin, myosin, and cardiac troponin T isoforms. C2C12 cells proliferate rapidly in high serum conditions and fuse under low serum conditions to form functional myotubes. The cell line does not exhibit tumorigenicity or metastatic potential. Though specific karyotype data is unavailable, C2C12 is known to have mutations related to CDKN2A gene in its immortalization process. It is resistant to lymphocytic choriomeningitis virus (LCMV), and primarily used to study muscle differentiation and regeneration.
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The C2C12 cell line holds broad utility in diverse applications due to its unique characteristics. It serves as a valuable model for assessing the impact of native and mutant cardiac and skeletal muscle contractile protein isoforms on myofilament function, enabling researchers to investigate the effects of specific protein modifications on muscle physiology. Moreover, C2C12 cells are well-suited for studies focusing on muscle cell differentiation, making them a preferred model for studying muscle development and regeneration. The utilization of C2C12-derived myotubes further enhances their usefulness in muscle-related research, particularly in areas requiring gene modification, facilitating advancements in understanding and manipulating muscle function at a molecular level.
