C2C12

C2C12 is a spontaneously immortalized murine myoblasts cell line derived from mouse skeletal muscle. During the process of fusion and differentiation into myotubes, the expression of myofilament proteins is initiated, encompassing multiple isoforms of cardiac and skeletal muscle troponin T. Fluorescence immunocytochemistry confirms the incorporation of cardiac troponin T isoforms into the myofibrils. Throughout the differentiation process, there is a notable transformation in the monolayer architecture as the cells transition from their initial fusiform or star-shaped morphology to elongated confluent cells, ultimately culminating in the formation of long, multinucleated myotubes. In their undifferentiated state, the myoblasts exhibit the presence of sarcomeric actin and myosin.

Why choose C2C12 from AcceGen?
Choosing C2C12 cells from AcceGen ensures high viability and quality, as they undergo rigorous quality control and advanced cryopreservation techniques. Additionally, AcceGen excludes mycoplasma contamination through PCR-based and luminescence-based mycoplasma assays, while also diligently monitoring for bacterial or fungal contaminations on a daily basis, providing researchers with reliable and uncontaminated cell lines for their experiments.