C57BL/6 Mouse Liver Sinusoidal Endothelial Cells

Description

C57BL/6 mouse liver sinusoidal endothelial cells (LSECs) are primary cells isolated from the liver tissue of pathogen-free adult C57BL/6 mice. These cells are involved in forming hepatic sinusoids and regulating the permeability of liver blood vessels. LSECs express LYVE-1, CD32b, Factor VIII, and are negative for CD31. They also express FcγRIIb2, PRRs, SRs, and TLRs, reflecting immune function. These cells are cryopreserved at early passage. These cells are applied in studies of liver fibrosis, steatohepatitis, and hepatic inflammation.

Application

• C57BL/6 mouse liver sinusoidal endothelial cells can be used in the research of liver regeneration. In the tubulogenesis assay, tubes and sprouts are generated by C57BL/6 mouse liver sinusoidal endothelial cells. When these cells are implanted in porous collagen scaffold, capillaries with lumen are formed. Liver sinusoidal endothelial cells can also form the spheroids expressing vascular endothelial growth factor-A and C (VEGF-A, VEGF-C) and hepatocyte growth factor (HGF), which are essential for liver regeneration.
  

• Additionally, C57BL/6 mouse liver sinusoidal endothelial cell is a valuable model for studying hepatic diseases or complications, such as non-alcoholic fatty liver disease (NAFLD), chronic liver injury, liver fibrosis, and liver metastasis. Moreover, these cells can also be applied in other diseases at extrahepatic sites, such as atherosclerosis, glomerular fibrotic nephrosis, and systemic lupus erythematosus (SLE) due to their role in the clearance of select substances from the blood.