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Primary Cells

Canine Aortic Endothelial Cells

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Canine Aortic Endothelial Cells (CnAOEC) are endothelial cells isolated from the dog aorta. CnAOEC are cryopreserved at second passage and can be passaged at least 16 doublings.
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Home | Primary Cells | Animal Primary Cells | Canine Primary Cells | Canine Aortic Endothelial Cells
Product Code

CnAOEC
Species

Canine
Cat.No

ABC-TC3263
Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.
Product Category Primary Cells
Size/Quantity

1 vial
Cell Type

Endothelial
Shipping Info

Dry Ice
Growth Conditions

37 ℃, 5% CO2
Source Organ

Aortic
Disease

Normal
Biosafety Level

1
Storage

Liquid Nitrogen
Product Type

Canine Primary Cells

Description

Canine primary aortic endothelial cells (CnAOEC) are derived from canine aortic tissue. Following the primary culture, these cells are cryopreserved. Endothelial cells serve as the natural barrier between blood and tissue, and are involved in the regulation of vasomotion, angiogenesis, and inflammation. They are metabolically active, producing autocrine and paracrine signaling molecules, supporting bidirectional molecular transport, and maintaining dynamic adhesion between cells and between cells and the extracellular matrix. In vitro, these cells should be avoided repeated freezing and thawing during culture.

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Citation

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Application

  • Dogs are considered valuable animal models for studying human diseases due to their physiological and genetic similarities to humans. They naturally develop cardiovascular conditions, such as heart failure, mitral valve disease, and arrhythmias, which progress in ways that closely resemble human disease. As such, CnAOECs (Canine Aortic Endothelial Cells) serve as a translational model for investigating the pathogenesis, progression, and treatment of human cardiovascular diseases. These cells can also be used to screen for candidate genes that may serve as novel endothelial cell markers.

Frequently Asked Questions

  • What are Canine Aortic Endothelial Cells (CAECs)?

    Canine Aortic Endothelial Cells (CAECs) are primary cells isolated from the inner lining of the aorta of dogs. These cells play a crucial role in vascular biology research, specifically for studying endothelial function and cardiovascular diseases.

  • What are CAECs used for in research?

    CAECs are used in various research applications including studies on vascular physiology, endothelial cell function, inflammation, angiogenesis, and the effects of drugs on vascular health. They are particularly valuable for modeling canine cardiovascular diseases and testing therapeutic interventions.

  • How do I ensure the optimal growth and health of CAECs in culture?

    To ensure optimal growth and health of CAECs, monitor cell confluency regularly and passage cells when they reach 70-80% confluency. Avoid over-confluency as it can lead to cell differentiation and altered function. Additionally, handle cells gently to prevent mechanical stress and maintain consistent environmental conditions.

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