Canine Aortic Endothelial Cells

Description

Canine Primary Aortic Endothelial Cells (CnAOEC) are derived from the aortic tissue of canines and cryopreserved after primary culture. These endothelial cells exhibit a typical cobblestone morphology and form a selective barrier between the bloodstream and vascular tissue. They express typical markers including CD31 (PECAM-1), von Willebrand factor (vWF), and vascular endothelial cadherin (VE-cadherin) and play essential roles in regulating vasomotion, angiogenesis, inflammation, and vascular homeostasis. Metabolically active, CnAOEC produce autocrine and paracrine signaling molecules, support bidirectional molecular transport, and maintain dynamic adhesion between cells as well as between cells and the extracellular matrix. This cell model is widely used in cardiovascular and vascular biology research. Repeated freezing and thawing should be avoided during culture to maintain cell viability and function. In vascular studies, these cells are sometimes compared with aortic stem populations, although CnAOEC represent differentiated endothelial cells rather than stem-like precursors.

Application

• Canine Primary Aortic Endothelial Cells (CnAOEC) are isolated from canine aortic tissue and cryopreserved following primary culture. These endothelial cells exhibit a characteristic cobblestone morphology and form a selective barrier between the bloodstream and vascular tissue. They express key endothelial markers including CD31 (PECAM-1), von Willebrand factor (vWF), and vascular endothelial cadherin (VE-cadherin). CnAOEC play essential roles in regulating vasomotion, angiogenesis, inflammation, and vascular homeostasis. Metabolically active, these cells produce autocrine and paracrine signaling molecules, facilitate bidirectional molecular transport, and maintain dynamic adhesion both between cells and between cells and the extracellular matrix. This cell model is widely used in cardiovascular and vascular biology research. To preserve cell viability and function, repeated freezing and thawing during culture should be avoided.