Primary Cells

Canine Dermal Fibroblasts

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Species

Canine

Cat.No

ABC-TC137W

Quality Control

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

Product Category Primary Cells
Size/Quantity

1 vial

Cell Type

Fibroblasts

Shipping Info

Dry Ice

Growth Conditions

37 °C, 5% CO2

Source Organ

Skin

Disease

Normal

Biosafety Level

1

Storage

Liquid Nitrogen

Product Type

Dermal Cells

Description

Canine Dermal Fibroblasts (CDFs) are primary spindle-shaped cells from healthy canine skin tissue, typically isolated from beagles or other breeds. In vitro, they maintain expression of collagen I, fibronectin, vimentin, and respond robustly to serum growth conditions, completing 70–80% confluency within 2–3 days. CDFs secrete key cytokines such as IL‑6, IL‑8, MCP‑1, VEGF‑A and SCF under inflammatory stimuli and are routinely used in PCR, Western blot, immunocytochemistry, and cell‑derivation protocols. Their consistency and multipassage stability make them ideal for studies in extracellular matrix remodeling, inflammation, wound healing, and biotherapeutic screening.

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Application

  • For research use only.

  • Canine Dermal Fibroblasts are widely used as in vitro models for extracellular matrix and wound healing research, assessing collagen and fibronectin secretion, proliferation under serum conditions, and response to pH or inflammatory stressors. They are instrumental in inflammation studies—measuring IL‑6/IL‑8/MCP‑1/VEGF release following exposure to inflammatory cytokines, allergens, or botanical extracts. They also support advanced applications in regenerative medicine, immunology, and translational veterinary biotechnology.

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