Canine Epididymal Epithelial Cells

Description

Canine Epididymal Epithelial Cells (CEECs) are primary cells isolated from the caput region of the canine epididymis, which retain a polarized pseudostratified morphology. They express tight junction proteins, including claudin‑1 and occludin, which are essential for maintaining the epithelial barrier. These cells are also involved in nutrient transport, as evidenced by the expression of monocarboxylate transporter 1 (MCT1). CEECs play a critical role in mechanotransduction, responding to fluid shear stress by activating primary cilia, which initiate intracellular calcium signaling and cytoskeletal remodeling. Moreover, they exhibit innate immune responses, activating Toll‑like receptor 3 (TLR3) and secreting proinflammatory cytokines, such as tumor necrosis factor‑α (TNF‑α) and monocyte chemoattractant protein‑1 (MCP‑1), upon exposure to viral mimics.

Application

• For research use only.

• Canine Epididymal Epithelial Cells (CEECs) are utilized to study epithelial barrier function, specifically nutrient uptake through MCT1-dependent pathways. They are also used in mechanotransduction research to examine primary cilia signaling and cytoskeletal changes under shear stress. CEECs provide an effective model for studying innate immune responses, especially TLR3 activation and subsequent cytokine production. Additionally, these cells support investigations into sperm–epithelial interactions, making them a valuable tool for reproductive and fertility research.