Canine Epididymal Epithelial Cells

Description

Canine Epididymal Epithelial Cells (CEECs) are primary cells isolated from the caput region of the canine epididymis, which retain a polarized pseudostratified morphology. They express tight junction proteins, including claudin‑1 and occludin, which are essential for maintaining the epithelial barrier. These cells are also involved in nutrient transport, as evidenced by the expression of monocarboxylate transporter 1 (MCT1). CEECs play a critical role in mechanotransduction, responding to fluid shear stress by activating primary cilia, which initiate intracellular calcium signaling and cytoskeletal remodeling. Moreover, they exhibit innate immune responses, activating Toll‑like receptor 3 (TLR3) and secreting proinflammatory cytokines, such as tumor necrosis factor‑α (TNF‑α) and monocyte chemoattractant protein‑1 (MCP‑1), upon exposure to viral mimics.